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Iron is increased in the brains of ageing mice lacking the neurofilament light gene

Citation

Vickers, JC and King, AE and McCormack, GH and Bindoff, AD and Adlard, PA, Iron is increased in the brains of ageing mice lacking the neurofilament light gene, PLoS ONE, 14, (10) Article e0224169. ISSN 1932-6203 (2019) [Refereed Article]


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DOI: doi:10.1371/journal.pone.0224169

Abstract

There has been strong interest in the role of metals in neurodegeneration, and how ageing may predispose the brain to related diseases such as Alzheimer's disease. Recent work has also highlighted a potential interaction between different metal species and various components of the cytoskeletal network in the brain, which themselves have a reported role in age-related degenerative disease and other neurological disorders. Neurofilaments are one such class of intermediate filament protein that have a demonstrated capacity to bind and utilise cation species. In this study, we investigated the consequences of altering the neurofilamentous network on metal ion homeostasis by examining neurofilament light (NFL) gene knockout mice, relative to wildtype control animals, at adulthood (5 months of age) and advanced age (22 months). Inductively coupled plasma mass spectroscopy demonstrated that the concentrations of iron (Fe), copper (Cu) and zinc (Zn) varied across brain regions and peripheral nerve samples. Zn and Fe showed statistically significant interactions between genotype and age, as well as between genotype and region, and Cu demonstrated a genotype and region interaction. The most substantial difference between genotypes was found in Fe in the older animals, where, across many regions examined, there was elevated Fe in the NFL knockout mice. This data indicates a potential relationship between the neurofilamentous cytoskeleton and the processing and/or storage of Fe through ageing.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Neurosciences
Research Field:Neurology and Neuromuscular Diseases
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Neurodegenerative Disorders Related to Ageing
UTAS Author:Vickers, JC (Professor James Vickers)
UTAS Author:King, AE (Professor Anna King)
UTAS Author:McCormack, GH (Mr Graeme McCormack)
UTAS Author:Bindoff, AD (Mr Aidan Bindoff)
ID Code:138059
Year Published:2019
Deposited By:Wicking Dementia Research and Education Centre
Deposited On:2020-03-23
Last Modified:2020-03-23
Downloads:0

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