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Stem cell proliferation is kept in check by the chromatin regulators Kismet/CHD7/CHD8 and Trr/MLL3/4


Gervais, L and van den Beek, M and Josserand, M and Salle, J and Stefanutti, M and Perdigoto, CN and Skorski, P and Mazouni, K and Marshall, OJ and Brand, AH and Schweisguth, F and Bardin, AJ, Stem cell proliferation is kept in check by the chromatin regulators Kismet/CHD7/CHD8 and Trr/MLL3/4, Developmental Cell, 49, (4) pp. 556-573. ISSN 1534-5807 (2019) [Refereed Article]


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Copyright 2019 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)

DOI: doi:10.1016/j.devcel.2019.04.033


Chromatin remodeling accompanies differentiation, however, its role in self-renewal is less well understood. We report that in Drosophila, the chromatin remodeler Kismet/CHD7/CHD8 limits intestinal stem cell (ISC) number and proliferation without affecting differentiation. Stem-cell-specific whole-genome profiling of Kismet revealed its enrichment at transcriptionally active regions bound by RNA polymerase II and Brahma, its recruitment to the transcription start site of activated genes and developmental enhancers and its depletion from regions bound by Polycomb, Histone H1, and heterochromatin Protein 1. We demonstrate that the Trithorax-related/MLL3/4 chromatin modifier regulates ISC proliferation, colocalizes extensively with Kismet throughout the ISC genome, and co-regulates genes in ISCs, including Cbl, a negative regulator of Epidermal Growth Factor Receptor (EGFR). Loss of kismet or trr leads to elevated levels of EGFR protein and signaling, thereby promoting ISC self-renewal. We propose that Kismet with Trr establishes a chromatin state that limits EGFR proliferative signaling, preventing tumor-like stem cell overgrowths.

Item Details

Item Type:Refereed Article
Keywords:Cbl, Drosophila midgut, EGFR, epigenetic, Kismet/CHD7/CHD8, proliferation control, Trr/MLL3/4, adult stem cells, chromatin regulators, tissue homeostasis
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Cellular nervous system
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Marshall, OJ (Dr Owen Marshall)
ID Code:138056
Year Published:2019
Web of Science® Times Cited:13
Deposited By:Menzies Institute for Medical Research
Deposited On:2020-03-22
Last Modified:2022-08-23
Downloads:18 View Download Statistics

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