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Transcranial contrast-enhanced ultrasound in the rat brain reveals substantial hyperperfusion acutely post-stroke

Citation

Premilovac, D and Blackwood, SJ and Ramsay, C and Keske, MA and Howells, DW and Sutherland, BA, Transcranial contrast-enhanced ultrasound in the rat brain reveals substantial hyperperfusion acutely post-stroke, Journal of Cerebral Blood Flow and Metabolism pp. 1-15. ISSN 0271-678X (2020) [Refereed Article]


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DOI: doi:10.1177/0271678X20905493

Abstract

Direct and real-time assessment of cerebral hemodynamics is key to improving our understanding of cerebral blood flow regulation in health and disease states such as stroke. While a number of sophisticated imaging platforms enable assessment of cerebral perfusion, most are limited either spatially or temporally. Here, we applied transcranial contrast-enhanced ultrasound (CEU) to measure cerebral perfusion in real-time through the intact rat skull before, during and after ischemic stroke, induced by intraluminal filament middle cerebral artery occlusion (MCAO). We demonstrate expected decreases in cortical and striatal blood volume, flow velocity and perfusion during MCAO. After filament retraction, blood volume and perfusion increased two-fold above baseline, indicative of acute hyperperfusion. Adjacent brain regions to the ischemic area and the contralateral hemisphere had increased blood volume during MCAO. We assessed our data using wavelet analysis to demonstrate striking vasomotion changes in the ischemic and contralateral cortices during MCAO and reperfusion. In conclusion, we demonstrate the application of CEU for real-time assessment of cerebral hemodynamics and show that the ischemic regions exhibit striking hyperemia post-MCAO. Whether this post-stoke hyperperfusion is sustained long-term and contributes to stroke severity is not known.

Item Details

Item Type:Refereed Article
Keywords:ischemic stroke, cerebral blood flow, contrast-enhanced ultrasound,middle cerebral artery occlusion, rat
Research Division:Medical and Health Sciences
Research Group:Medical Physiology
Research Field:Systems Physiology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Cardiovascular System and Diseases
UTAS Author:Premilovac, D (Dr Dino Premilovac)
UTAS Author:Ramsay, C (Mr Ciaran Ramsay)
UTAS Author:Howells, DW (Professor David Howells)
UTAS Author:Sutherland, BA (Dr Brad Sutherland)
ID Code:138031
Year Published:2020
Deposited By:Medicine
Deposited On:2020-03-20
Last Modified:2020-03-20
Downloads:0

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