Premilovac et al_Manuscript_JCBFM_revision clean w figs.pdf (1.17 MB)
Transcranial contrast-enhanced ultrasound in the rat brain reveals substantial hyperperfusion acutely post-stroke
journal contribution
posted on 2023-05-20, 11:55 authored by Dino PremilovacDino Premilovac, Blackwood, SJ, Ramsay, C, Keske, MA, David Howells, Brad SutherlandBrad SutherlandDirect and real-time assessment of cerebral hemodynamics is key to improving our understanding of cerebral blood flow regulation in health and disease states such as stroke. While a number of sophisticated imaging platforms enable assessment of cerebral perfusion, most are limited either spatially or temporally. Here, we applied transcranial contrast-enhanced ultrasound (CEU) to measure cerebral perfusion in real-time through the intact rat skull before, during and after ischemic stroke, induced by intraluminal filament middle cerebral artery occlusion (MCAO). We demonstrate expected decreases in cortical and striatal blood volume, flow velocity and perfusion during MCAO. After filament retraction, blood volume and perfusion increased two-fold above baseline, indicative of acute hyperperfusion. Adjacent brain regions to the ischemic area and the contralateral hemisphere had increased blood volume during MCAO. We assessed our data using wavelet analysis to demonstrate striking vasomotion changes in the ischemic and contralateral cortices during MCAO and reperfusion. In conclusion, we demonstrate the application of CEU for real-time assessment of cerebral hemodynamics and show that the ischemic regions exhibit striking hyperemia post-MCAO. Whether this post-stoke hyperperfusion is sustained long-term and contributes to stroke severity is not known.
Funding
Royal Hobart Hospital Research Foundation
History
Publication title
Journal of Cerebral Blood Flow and MetabolismPagination
1-15ISSN
0271-678XDepartment/School
Tasmanian School of MedicinePublisher
Lippincott Williams & WilkinsPlace of publication
530 Walnut St, Philadelphia, USA, Pa, 19106-3621Rights statement
Copyright 2020 The AuthorsRepository Status
- Open