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Understanding mechanisms of salinity tolerance in barley by proteomic and biochemical analysis of near-isogenic lines

Citation

Zhu, J and Fan, Y and Shabala, S and Li, C and Lv, C and Guo, B and Xu, R and Zhou, M, Understanding mechanisms of salinity tolerance in barley by proteomic and biochemical analysis of near-isogenic lines, International Journal of Molecular Sciences, 21, (4) Article 1516. ISSN 1422-0067 (2020) [Refereed Article]


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Copyright Statement

Copyright 2020 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3390/ijms21041516

Abstract

Salt stress is one of the major environmental factors impairing crop production. In our previous study, we identified a major QTL for salinity tolerance on chromosome 2H on barley (Hordeum vulgare L.). For further investigation of the mechanisms responsible for this QTL, two pairs of near-isogenic lines (NILs) differing in this QTL were developed. Sensitive NILs (N33 and N53) showed more severe damage after exposure to 300 mM NaCl than tolerant ones (T46 and T66). Both tolerant NILs maintained significantly lower Na+ content in leaves and much higher K+ content in the roots than sensitive lines under salt conditions, thus indicating the presence of a more optimal Na+/K+ ratio in plant tissues. Salinity stress caused significant accumulation of H2O2, MDA, and proline in salinity-sensitive NILs, and a greater enhancement in antioxidant enzymatic activities at one specific time or tissues in tolerant lines. One pair of NILs (N33 and T46) were used for proteomic studies using two-dimensional gel electrophoresis. A total of 53 and 51 differentially expressed proteins were identified through tandem mass spectrometry analysis in the leaves and roots, respectively. Proteins which are associated with photosynthesis, reactive oxygen species (ROS) scavenging, and ATP synthase were found to be specifically upregulated in the tolerant NIL. Proteins identified in this study can serve as a useful resource with which to explore novel candidate genes for salinity tolerance in barley.

Item Details

Item Type:Refereed Article
Keywords:barley (Hordeum vulgare L.), near-isogenic lines, salinity tolerance, photosynthesis, ROS scavenging, ATP synthase, salinity
Research Division:Agricultural and Veterinary Sciences
Research Group:Crop and Pasture Production
Research Field:Agro-ecosystem Function and Prediction
Objective Division:Plant Production and Plant Primary Products
Objective Group:Winter Grains and Oilseeds
Objective Field:Barley
UTAS Author:Zhu, J (Miss Juan Zhu)
UTAS Author:Fan, Y (Miss Yun Fan)
UTAS Author:Shabala, S (Professor Sergey Shabala)
UTAS Author:Zhou, M (Professor Meixue Zhou)
ID Code:137711
Year Published:2020
Deposited By:TIA - Research Institute
Deposited On:2020-02-28
Last Modified:2020-07-27
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