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An acid up-regulated surface protein of Lactobacillus paracasei strain GCRL 46 is phylogenetically related to the secreted glucan-(GpbB) and immunoglobulin-binding (SibA) protein of pathogenic streptococci

Citation

Pepper, SJ and Britz, ML, An acid up-regulated surface protein of Lactobacillus paracasei strain GCRL 46 is phylogenetically related to the secreted glucan-(GpbB) and immunoglobulin-binding (SibA) protein of pathogenic streptococci, International Journal of Molecular Sciences, 20, (7) Article 1610. ISSN 1661-6596 (2019) [Refereed Article]


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Copyright Statement

Copyright 2019 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3390/ijms20071610

Abstract

Bacterial cell wall hydrolases, including amidases and peptidases, play a critical role in peptidoglycan turnover during growth, impacting daughter cell separation, and cell death, through autolysis. When exploring the regulation of protein expression across the growth cycle of an acid-resistant strain of Lactobacillus paracasei, GCRL 46, we observed temporal up-regulation of proteins in the 4045 kDa molecular weight range for whole-cell extracts when culturing in fermenters at a controlled pH of 4.0 versus optimum growth pH of 6.3. Up-regulation of proteins in this size range was not detected in SDS-PAGE gels of the cytosolic fraction, but was routinely detected following growth at low pH in whole cells and cell debris obtained after bead beating and centrifugation, indicating a cell surface location. N-terminal sequencing and in silico analyses showed sequence similarity with proteins in the L. casei group (L. casei, L. paracasei and L. rhamnosus) which were variously annotated as uncharacterized proteins, surface antigens, possible TrsG proteins, CHAP (cysteine, histidine-dependent amidohydrolases/peptidases)-domain proteins or putative peptidoglycan d,l-endopeptidase due to the presence of a CwlO domain. This protein is a homologue of the p40 (Msp2) secreted protein of L. rhamnosus LGG, which is linked to probiotic functionality in this species, and is phylogenetically related to structurally-similar proteins found in Enterococcus, Streptococcus and Bifidobacterium species, including the glucan-binding (GbpB), surface antigen (SagA) proteins detected in pathogenic group A streptococci species as secreted, immunoglobulin-binding (SibA) proteins (also named PcsB). Three-dimensional (3D) modelling predicted structural similarities in the CHAP proteins from the L. casei group and streptococcal species, indicating retention of overall architecture despite sequence divergence, and an implied retention of function during evolution. A phylogenetically-related hydrolase also contained the CwlO domain with a NLPC_P60 domain, and showed similar overall but distinct architecture to the CHAP proteins. We concluded that the surface-located, CHAP protein in L. casei is up-regulated during long-term exposure to acidic conditions during growth but not during acid shock.

Item Details

Item Type:Refereed Article
Keywords:Lactobacillus casei, Streptococcus, SibA, CHAP, PcsB, cell-wall hydrolase, acid resistance
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbiology not elsewhere classified
Objective Division:Manufacturing
Objective Group:Dairy products
Objective Field:Dairy products not elsewhere classified
UTAS Author:Britz, ML (Professor Margaret Britz)
ID Code:137428
Year Published:2019
Web of Science® Times Cited:6
Deposited By:TIA - Research Institute
Deposited On:2020-02-12
Last Modified:2020-04-09
Downloads:4 View Download Statistics

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