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Possibilities and limitations of the sequential injection chromatography technique for the determination of anticoccidial agents in water, pharmaceutical formulations and feed
This paper explores the potential of applying reversed-phase sequential injection chromatography (SIC) to determine the anticoccidial agents Lasalocid and Toltrazuril in various matrices including ground water, pharmaceutical formulations and feed. SIC was performed by connecting a 25 × 4.6 mm monolithic C18 column to a 2 m long pathlength capillary flow cell, where the usage of a flow cell lowers the detection limit compared to a conventional short-distance flow cell, providing a simple detection system for these two compounds which are initially poorly UV absorbents.
The proposed set-up provides a high injection throughput of 12 h− 1, as well as a limit of detection of 0.019 and 0.010 mg/L for Toltrazuril and Lasalocid, respectively. The repeatabilities obtained (n = 10) were lower than 2% and 4% for Toltrazuril and Lasalocid, respectively.
History
Publication title
Microchemical JournalVolume
98Pagination
190-199ISSN
0026-265XDepartment/School
School of Natural SciencesPublisher
ElsevierPlace of publication
Po Box 211, Amsterdam, Netherlands, 1000 AeRights statement
© 2011 Elsevier B.V. All rights reserved.Repository Status
- Restricted