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High-dose vitamin C supplementation increases skeletal muscle vitamin C concentration and SVCT2 transporter expression but does not alter redox status in healthy males

Citation

Mason, SA and Baptista, R and Della Gatta, PA and Yousif, A and Russell, AP and Wadley, GD, High-dose vitamin C supplementation increases skeletal muscle vitamin C concentration and SVCT2 transporter expression but does not alter redox status in healthy males, Free Radical Biology and Medicine, 77 pp. 130-138. ISSN 0891-5849 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Elsevier Inc. All rights reserved.

DOI: doi:10.1016/j.freeradbiomed.2014.09.013

Abstract

Antioxidant vitamin C (VC) supplementation is of potential clinical benefit to individuals with skeletal muscle oxidative stress. However, there is a paucity of data reporting on the bioavailability of high-dose oral VC in human skeletal muscle. We aimed to establish the time course of accumulation of VC in skeletal muscle and plasma during high-dose VC supplementation in healthy individuals. Concurrently we investigated the effects of VC supplementation on expression levels of the key skeletal muscle VC transporter sodium-dependent vitamin C transporter 2 (SVCT2) and intramuscular redox and mitochondrial measures. Eight healthy males completed a randomized placebo-controlled, crossover trial involving supplementation with ascorbic acid (2 × 500 mg/day) over 42 days. Participants underwent muscle and blood sampling on days 0, 1, 7, and 42 during each treatment. VC supplementation significantly increased skeletal muscle VC concentration after 7 days, which was maintained at 42 days (VC 3.0 ± 0.2 (mean ± SEM) to 3.9 ± 0.4 mg/100 g wet weight (ww) versus placebo 3.1 ± 0.3 to 2.9 ± 0.2 mg/100 g ww, p = 0.001). Plasma VC increased after 1 day, which was maintained at 42 days (VC 61.0 ± 6.1 to 111.5 ± 10.4 µmol/L versus placebo 60.7 ± 5.3 to 59.2 ± 4.8 µmol/L, p < 0.001). VC supplementation significantly increased skeletal muscle SVCT2 protein expression (main treatment effect p = 0.006) but did not alter skeletal muscle redox measures or citrate synthase activity. A main finding of our study was that 7 days of high-dose VC supplementation was required to significantly increase skeletal muscle vitamin C concentration in healthy males. Our findings implicate regular high-dose vitamin C supplementation as a means to safely increase skeletal muscle vitamin C concentration without impairing intramuscular ascorbic acid transport, antioxidant concentrations, or citrate synthase activity.

Item Details

Item Type:Refereed Article
Keywords:vitamin C, skeletal muscle, antioxidant, reactive oxygen species, sodium-dependent vitamin C transporter2, free radicals
Research Division:Health Sciences
Research Group:Health services and systems
Research Field:Health services and systems not elsewhere classified
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Yousif, A (Dr Adel Yousif)
ID Code:136765
Year Published:2014
Web of Science® Times Cited:16
Deposited By:Agriculture and Food Systems
Deposited On:2020-01-17
Last Modified:2020-04-21
Downloads:0

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