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Lactobacillus acidophilus DDS-1 modulates intestinal-specific microbiota, short-chain fatty acid and immunological profiles in aging mice

Citation

Vemuri, R and Gundamaraju, R and Shinde, T and Perera, AP and Basheer, W and Southam, B and Gondalia, SV and Karpe, AV and Beale, DJ and Tristram, S and Ahuja, KDK and Ball, M and Martoni, CJ and Eri, R, Lactobacillus acidophilus DDS-1 modulates intestinal-specific microbiota, short-chain fatty acid and immunological profiles in aging mice, Nutrients, 11, (6) pp. 1-23. ISSN 2072-6643 (2019) [Refereed Article]


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DOI: doi:10.3390/nu11061297

Abstract

Distribution of the microbiota varies according to the location in the gastrointestinal (GI) tract. Thus, dysbiosis during aging may not be limited to faecal microbiota and extend to the other parts of the GI tract, especially the cecum and colon. Lactobacillus acidophilus DDS-1, a probiotic strain, has been shown to modulate faecal microbiota and its associated metabolic phenotype in aging mice. In the present study, we investigated the effect of L. acidophilus DDS-1 supplementation on caecal- and mucosal-associated microbiota, short-chain fatty acids (SCFAs) and immunological profiles in young and aging C57BL/6J mice. Besides differences in the young and aging control groups, we observed microbial shifts in caecal and mucosal samples, leading to an alteration in SCFA levels and immune response. DDS-1 treatment increased the abundances of beneficial bacteria such as Akkermansia spp. and Lactobacillus spp. more effectively in caecal samples than in mucosal samples. DDS-1 also enhanced the levels of butyrate, while downregulating the production of inflammatory cytokines (IL-6, IL-1β, IL-1α, MCP-1, MIP-1α, MIP-1β, IL-12 and IFN-γ) in serum and colonic explants. Our findings suggest distinct patterns of intestinal microbiota, improvements in SCFA and immunological profiles with DDS-1 supplementation in aging mice.

Item Details

Item Type:Refereed Article
Research Division:Medical and Health Sciences
Research Group:Nutrition and Dietetics
Research Field:Nutritional Physiology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Digestive System Disorders
UTAS Author:Vemuri, R (Mr Ravichandra Vemuri)
UTAS Author:Gundamaraju, R (Mr Rohit Gundamaraju)
UTAS Author:Shinde, T (Ms Tanvi Shinde)
UTAS Author:Perera, AP (Mrs Agampodi Perera)
UTAS Author:Basheer, W (Dr Waheedha Basheer)
UTAS Author:Southam, B (Mr Benjamin Southam)
UTAS Author:Tristram, S (Dr Stephen Tristram)
UTAS Author:Ahuja, KDK (Dr Kiran Ahuja)
UTAS Author:Eri, R (Associate Professor Raj Eri)
ID Code:136658
Year Published:2019
Deposited By:Health Sciences
Deposited On:2020-01-13
Last Modified:2020-01-13
Downloads:0

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