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Microhair on the adaxial leaf surface of salt secreting halophytic Oryza coarctata Roxb. show distinct morphotypes: Isolation for molecular and functional analysis

Citation

Rajakani, R and Sellamuthu, G and Saravanakumar, V and Kannappan, S and Shabala, L and Meinke, H and Chen, Z and Zhou, M and Parida, A and Shabala, S and Venkataraman, G, Microhair on the adaxial leaf surface of salt secreting halophytic Oryza coarctata Roxb. show distinct morphotypes: Isolation for molecular and functional analysis, Plant Science, 285 pp. 248-257. ISSN 0168-9452 (2019) [Refereed Article]


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DOI: doi:10.1016/j.plantsci.2019.05.004

Abstract

Halophytic Oryza coarctata is a good model system to examine mechanisms of salinity tolerance in rice. O. coarctata leaves show the presence of microhairs in adaxial leaf surface furrows that secrete salt under salinity. However, detailed molecular and physiological studies of O. coarctata microhairs are limited due to their relative inaccessibility. This work presents a detailed characterization of O. coarctata leaf features. O. coarctata has two types of microhairs on the adaxial leaf surface: longer microhairs (three morphotypes) lining epidermal furrow walls and shorter microhairs (reported first time) arising from bulliform cells. Microhair morphotypes include (i) finger-like, tubular structures, (ii) tubular hairs with bilobed and flattened heads and (iii) bi-or trifurcated hairs. The unicellular nature of microhairs was confirmed by propidium iodide (PI) staining. An efficient method for the isolation and enrichment of O. coarctata microhairs is presented (yield averaging ∼2  105/g leaf tissue). The robustness of the microhair isolation procedure was confirmed by subsequent viability staining (PI), total RNA isolation and RT-PCR amplification of O. coarctata trichome-specific WUSCHEL-related homeobox 3B (OcWox3B) and transporter gene-specific cDNA sequences. The present microhair isolation work from O. coarctata paves the way for examining genes involved in ion secretion in this halophytic wild rice model.

Item Details

Item Type:Refereed Article
Keywords:salinity, microhair, bulliform cells, propidium iodide, percoll gradient, RT-PCR
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Summer Grains and Oilseeds
Objective Field:Rice
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Meinke, H (Professor Holger Meinke)
UTAS Author:Zhou, M (Professor Meixue Zhou)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:136643
Year Published:2019
Deposited By:Agriculture and Food Systems
Deposited On:2020-01-11
Last Modified:2020-01-14
Downloads:0

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