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Extracellular spermine triggers a rapid intracellular phosphatidic acid response in arabidopsis, involving PLDδ activation and stimulating ion flux

Citation

Zarza, X and Shabala, L and Fujita, M and Shabala, S and Haring, MA and Tiburcio, AF and Munnik, T, Extracellular spermine triggers a rapid intracellular phosphatidic acid response in arabidopsis, involving PLDδ activation and stimulating ion flux, Frontiers in Plant Science, 10 Article 601. ISSN 1664-462X (2019) [Refereed Article]


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Copyright Statement

Copyright 2019 Zarza, Shabala, Fujita, Shabala, Haring, Tiburcio and Munnik. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3389/fpls.2019.00601

Abstract

Polyamines, such as putrescine (Put), spermidine (Spd), and spermine (Spm), are low-molecular-weight polycationic molecules found in all living organisms. Despite the fact that they have been implicated in various important developmental and adaptative processes, their mode of action is still largely unclear. Here, we report that Put, Spd, and Spm trigger a rapid increase in the signaling lipid, phosphatidic acid (PA) in Arabidopsis seedlings but also mature leaves. Using time-course and dose-response experiments, Spm was found to be the most effective; promoting PA responses at physiological (low μM) concentrations. In seedlings, the increase of PA occurred mainly in the root and partly involved the plasma membrane polyamine-uptake transporter (PUT), RMV1. Using a differential 32Pi-labeling strategy combined with transphosphatidylation assays and T-DNA insertion mutants, we found that phospholipase D (PLD), and in particular PLDδ was the main contributor of the increase in PA. Measuring non-invasive ion fluxes (MIFE) across the root plasma membrane of wild type and pldδ-mutant seedlings, revealed that the formation of PA is linked to a gradual- and transient efflux of K+. Potential mechanisms of how PLDδ and the increase of PA are involved in polyamine function is discussed.

Item Details

Item Type:Refereed Article
Keywords:phospholipase D (PLD), phosphatidic acid (PA), lipid signaling, polyamines (putrescine, spermidine, spermine), phospholipids, MIFE
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other Plant Production and Plant Primary Products
Objective Field:Plant Production and Plant Primary Products not elsewhere classified
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:136628
Year Published:2019
Web of Science® Times Cited:1
Deposited By:Agriculture and Food Systems
Deposited On:2020-01-11
Last Modified:2020-04-06
Downloads:1 View Download Statistics

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