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Plants grown in parafilm-wrapped petri dishes are stressed and possess altered gene expression profile

Citation

Xu, L and Li, S and Shabala, S and Jian, T and Zhang, W, Plants grown in parafilm-wrapped petri dishes are stressed and possess altered gene expression profile, Frontiers in Plant Science, 10 Article 637. ISSN 1664-462X (2019) [Refereed Article]


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Copyright Statement

Copyright 2019 Xu, Li, Shabala, Jian and Zhang. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3389/fpls.2019.00637

Abstract

Arabidopsis is used as a model species in numerous physiological and genetic studies. Most of them employ parafilm-wrapped sterile culture. Here we demonstrate that this method is prone to potential artifacts and can lead to erroneous conclusions. We compared the effect of different sealing methods including air-permeable paper tape and traditional parafilm on Arabidopsis seedling growth, root development and gene expression network. Although seedlings grown in Petri dishes after 1 week sealed with paper tape showed a similar growth phenotype to that of parafilm-sealed seedlings, more than 700 differentially expressed genes (DEG) were found, including stress and nutrition-responsive genes. In addition, more H2O2 was accumulated in the tissues of parafilm-sealed plants. After 14 days of growth, paper tape-sealed plants grew much better than parafilm-sealed ones and accumulated higher chlorophyll content, with 490 DEGs found. After 3 weeks of growth, paper tape-sealed plants had higher chlorophyll and better growth compared to parafilm-sealed ones; and only 10 DEGs were found at this stage. Thus, the obvious phenotype observed at the latter stage was a result of differential gene expression at earlier time points, mostly of defense, abiotic stress, nutrition, and phytohormone-responsive genes. More O2 content was detected inside paper tape-sealed Petri dishes at early growth stage (7 days), and distinct difference in the CO2 content was observed between parafilm-sealed and paper tape-sealed Petri dishes. Furthermore, the carbon source also influenced seedlings growth with different sealing methods. In conclusion, conventional sealing using parafilm was not the optimal choice, most likely because of the limited gas exchange and a consequent stress caused to plants.

Item Details

Item Type:Refereed Article
Keywords:Arabidopsis, transcriptome analysis, gas exchange, abiotic stress, sterile culture, growth, ethylene, hypoxia
Research Division:Biological Sciences
Research Group:Plant Biology
Research Field:Plant Physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other Plant Production and Plant Primary Products
Objective Field:Plant Production and Plant Primary Products not elsewhere classified
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:136627
Year Published:2019
Deposited By:Agriculture and Food Systems
Deposited On:2020-01-11
Last Modified:2020-05-22
Downloads:0

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