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Auxin stimulates Cl- uptake by oat coleoptiles
journal contribution
posted on 2023-05-16, 11:07 authored by Babourina, OK, Sergey ShabalaSergey Shabala, Ian NewmanIan NewmanThe effects of auxin on net ion fluxes near parenchyma of oat coleoptiles were studied using the non-invasive MIFE system to measure specific ion fluxes using ion selective microelectrodes. Application of 10 μM 1-naphthaleneacetic acid (NAA) for 3 h caused doubling of coleoptile segment growth, without changing the pH of the unbuffered bathing solution from pH 5.4 during that time. Short term experiments revealed that auxin led to an immediate three-fold increase of chloride influx to 1200 nmol m-2 s-1, maintained for at least 1 h. In the first minutes after auxin application, proton fluxes were small (-25 nmol m-2 s-1, an efflux) and tended to decrease, whereas potassium and calcium fluxes changed little, fluctuating from - 100 to 0 nmol m-2 s-1 and from - 15 to 0 nmol m-2 s-1, respectively. It is suggested that one target of auxin action in plant cells is the plasma membrane chloride transport system mediating increased chloride uptake.
History
Publication title
Annals of BotanyVolume
82Pagination
331-336ISSN
0305-7364Department/School
School of Natural SciencesPublisher
Annals of Botany CompanyPlace of publication
Great Clarendon St, Oxford, England, Ox2 6DpRepository Status
- Restricted