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Redshifted and near-infrared active analog pigments based upon archaerhodopsin-3

Citation

Ganapathy, S and Kratz, S and Chen, Q and Hellingwerf, KJ and de Groot, HJM and Rothschild, KJ and de Grip, WJ, Redshifted and near-infrared active analog pigments based upon archaerhodopsin-3, Photochemistry and Photobiology, 95, (4) pp. 959-968. ISSN 0031-8655 (2019) [Refereed Article]


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Copyright 2019 The Authors Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1111/php.13093

Abstract

Archaerhodopsin‐3 (AR3) is a member of the microbial rhodopsin family of hepta‐helical transmembrane proteins, containing a covalently bound molecule of all‐trans retinal as a chromophore. It displays an absorbance band in the visible region of the solar spectrum (λmax 556 nm) and functions as a light‐driven proton pump in the archaeon Halorubrum sodomense. AR3 and its mutants are widely used in neuroscience as optogenetic neural silencers and in particular as fluorescent indicators of transmembrane potential. In this study, we investigated the effect of analogs of the native ligand all‐trans retinal A1 on the spectral properties and proton‐pumping activity of AR3 and its single mutant AR3 (F229S). While, surprisingly, the 3‐methoxyretinal A2 analog did not redshift the absorbance maximum of AR3, the analogs retinal A2 and 3‐methylamino‐16‐nor‐1,2,3,4‐didehydroretinal (MMAR) did generate active redshifted AR3 pigments. The MMAR analog pigments could even be activated by near‐infrared light. Furthermore, the MMAR pigments showed strongly enhanced fluorescence with an emission band in the near‐infrared peaking around 815 nm. We anticipate that the AR3 pigments generated in this study have widespread potential for near‐infrared exploitation as fluorescent voltage‐gated sensors in optogenetics and artificial leafs and as proton pumps in bioenergy‐based applications.

Item Details

Item Type:Refereed Article
Keywords:archaerhodopsin‐3, near-infrared active analog pigments, hepta‐helical transmembrane proteins
Research Division:Physical Sciences
Research Group:Optical Physics
Research Field:Photonics, Optoelectronics and Optical Communications
Objective Division:Manufacturing
Objective Group:Industrial Chemicals and Related Products
Objective Field:Industrial Chemicals and Related Products not elsewhere classified
UTAS Author:Kratz, S (Dr Svenja Kratz)
ID Code:135820
Year Published:2019
Web of Science® Times Cited:5
Deposited By:Office of the School of Creative Arts and Media
Deposited On:2019-11-15
Last Modified:2019-12-09
Downloads:4 View Download Statistics

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