Naresh, R and Song, Y and Hampson, DJ, The intestinal spirochete Brachyspira pilosicoli attaches to cultured Caco-2 cells and induces pathological changes, PLoS One, 4, (12) Article e8352. ISSN 1932-6203 (2009) [Refereed Article]
Copyright 2009 Naresh et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Methodology/Principal Findings: Four strains of B. pilosicoli were incubated at a high multiplicity of infection with monolayers of a human colonic adenocarcinoma cell line (Caco-2 cells). One strain isolated from a pig (95/1000) and one from a human (WesB) attached to the monolayers. Colonization increased with time, with the Caco-2 cell junctions being the initial targets of attachment. By electron microscopy, individual spirochete cells could be seen to have one cell end invaginated into the Caco-2 cell membranes, with the rest of the spirochete draped over the Caco-2 cell surface. After 6 h incubation, the monolayer was covered with a layer of spirochetes. Colonized monolayers demonstrated a time-dependent series of changes: staining with labelled phalloidin identified accumulation of actin at the cell junctions; ZO-1 staining revealed a loss of Caco-2 tight junction integrity; and Hoechst staining showed condensation and fragmentation of nuclear material consistent with apoptosis. Using quantitative reverse transcription PCR, the colonized monolayers demonstrated a significant up-regulation of interleukin-1beta (IL-1beta) and IL-8 expression. B. pilosicoli sonicates caused significant up-regulation of IL-1beta, TNF-alpha, and IL-6, but culture supernatants and non-pathogenic Brachyspira innocens did not alter cytokine expression.
Conclusions/Significance: The changes induced in the Caco-2 cells provide evidence that B. pilosicoli has pathogenic potential, and give insights into the likely in vivo pathogenesis.
|Item Type:||Refereed Article|
|Keywords:||Brachyspira pilosicoli, in vivo, pathogenesis|
|Research Division:||Medical and Health Sciences|
|Research Group:||Medical Microbiology|
|Research Field:||Medical Bacteriology|
|Objective Group:||Other Health|
|Objective Field:||Health not elsewhere classified|
|UTAS Author:||Song, Y (Dr Yong Song)|
|Web of Science® Times Cited:||26|
|Deposited By:||Menzies Institute for Medical Research|
|Downloads:||4 View Download Statistics|
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