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The intestinal spirochete Brachyspira pilosicoli attaches to cultured Caco-2 cells and induces pathological changes
Citation
Naresh, R and Song, Y and Hampson, DJ, The intestinal spirochete Brachyspira pilosicoli attaches to cultured Caco-2 cells and induces pathological changes, PLoS One, 4, (12) Article e8352. ISSN 1932-6203 (2009) [Refereed Article]
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Copyright Statement
Copyright 2009 Naresh et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
DOI: doi:10.1371/journal.pone.0008352
Abstract
Methodology/Principal Findings: Four strains of B. pilosicoli were incubated at a high multiplicity of infection with monolayers of a human colonic adenocarcinoma cell line (Caco-2 cells). One strain isolated from a pig (95/1000) and one from a human (WesB) attached to the monolayers. Colonization increased with time, with the Caco-2 cell junctions being the initial targets of attachment. By electron microscopy, individual spirochete cells could be seen to have one cell end invaginated into the Caco-2 cell membranes, with the rest of the spirochete draped over the Caco-2 cell surface. After 6 h incubation, the monolayer was covered with a layer of spirochetes. Colonized monolayers demonstrated a time-dependent series of changes: staining with labelled phalloidin identified accumulation of actin at the cell junctions; ZO-1 staining revealed a loss of Caco-2 tight junction integrity; and Hoechst staining showed condensation and fragmentation of nuclear material consistent with apoptosis. Using quantitative reverse transcription PCR, the colonized monolayers demonstrated a significant up-regulation of interleukin-1beta (IL-1beta) and IL-8 expression. B. pilosicoli sonicates caused significant up-regulation of IL-1beta, TNF-alpha, and IL-6, but culture supernatants and non-pathogenic Brachyspira innocens did not alter cytokine expression.
Conclusions/Significance: The changes induced in the Caco-2 cells provide evidence that B. pilosicoli has pathogenic potential, and give insights into the likely in vivo pathogenesis.
Item Details
Item Type: | Refereed Article |
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Keywords: | Brachyspira pilosicoli, in vivo, pathogenesis |
Research Division: | Biomedical and Clinical Sciences |
Research Group: | Medical microbiology |
Research Field: | Medical bacteriology |
Objective Division: | Health |
Objective Group: | Other health |
Objective Field: | Other health not elsewhere classified |
UTAS Author: | Song, Y (Dr Yong Song) |
ID Code: | 135803 |
Year Published: | 2009 |
Web of Science® Times Cited: | 28 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2019-11-14 |
Last Modified: | 2019-12-16 |
Downloads: | 15 View Download Statistics |
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