Song, Y and Frey, B and Hampson, DJ, The use of ELISAs for monitoring exposure of pig herds to Brachyspira hyodysenteriae, BMC Veterinary Research, 8 Article 6. ISSN 1746-6148 (2012) [Refereed Article]
Copyright 2012 Song et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Results: Levels of circulating IgG and IgM reacting with WC spirochaete preparations and recombinant Bhlp29.7 peaked 4-6 weeks post-infection in the experimentally challenged pigs, and remained elevated in the present study. In a cohort of pigs on an infected farm levels of antibody directed against both antigens showed a progressive increase with time. However, other than for the level of IgG against WC antigen, a significant increase in antibody levels also was observed in a cohort of pigs on a non-infected farm. In addition, assays using meat juice had 100% specificity and equivalent sensitivity to those based on serum, and likewise the best performance was achieved using the WC IgG ELISA.
Conclusions: IgG ELISAs using either WC or Bhlp29.7 as plate-coating antigens were shown to be useful for monitoring the dynamics of B. hyodysenteriae infection in grower pigs. Of the two antigens, the WC preparation tended to give better discrimination between pigs from infected and non-infected farms. Testing of meat juice was shown to have potential for identifying infected herds.
|Item Type:||Refereed Article|
|Keywords:||Antibody profiles, Brachyspira hyodysenteriae, ELISA, meat juice, swine dysentery|
|Research Division:||Agricultural, Veterinary and Food Sciences|
|Research Group:||Veterinary sciences|
|Research Field:||Veterinary medicine (excl. urology)|
|Objective Group:||Clinical health|
|Objective Field:||Clinical health not elsewhere classified|
|UTAS Author:||Song, Y (Dr Yong Song)|
|Web of Science® Times Cited:||6|
|Deposited By:||Menzies Institute for Medical Research|
|Downloads:||6 View Download Statistics|
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