Lavin, T and Song, Y and Bakker, AJ and McLean, CJ and Macdonald, WA and Noble, PB and Berry, CA and Pillow, JJ and Pinniger, GJ, Developmental changes in diaphragm muscle function in the preterm and postnatal lamb, Pediatric Pulmonology, 48, (7) pp. 640-648. ISSN 8755-6863 (2013) [Refereed Article]
Copyright 2013 Wiley Periodicals, Inc.
Methods: Lambs were studied at 75, 100, 125, 145, 154, 168, and 200 days postconceptional age (term = 147 days). Lambs were euthanized (sodium pentobarbitone, 100 mg/kg) either at delivery or immediately prior to post-mortem for postnatal lambs. Contractile function was assessed on longitudinal strips of intact muscle fibers and the remaining tissue frozen in liquid nitrogen for analysis of myosin heavy chain (MHC) mRNA expression and protein content.
Results: Fetal development of diaphragm function was characterized by a significant increase in maximum specific force, increased susceptibility to fatigue, reduced twitch contraction times, and a progressive increase in MHCI and MHCII protein content. Postnatally, there was a progressive decrease in the susceptibility to fatigue that coincided with an increase in the MHC I:II protein ratio.
Conclusion: These data indicate that the functional capacity of the diaphragm varies with maturational age and may be an important determinant of the susceptibility to preterm respiratory failure.
|Item Type:||Refereed Article|
|Keywords:||respiratory muscle, ventilation, skeletal muscle, fatigue, ontogeny|
|Research Division:||Biomedical and Clinical Sciences|
|Research Group:||Cardiovascular medicine and haematology|
|Research Field:||Respiratory diseases|
|Objective Group:||Clinical health|
|Objective Field:||Clinical health not elsewhere classified|
|UTAS Author:||Song, Y (Dr Yong Song)|
|Web of Science® Times Cited:||9|
|Deposited By:||Menzies Institute for Medical Research|
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