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Influence of antenatal glucocorticoid on preterm lamb diaphragm

Citation

Mahzabin, T and Pillow, JJ and Pinniger, GJ and Bakker, AJ and Noble, PB and White, RB and Karisnan, K and Song, Y, Influence of antenatal glucocorticoid on preterm lamb diaphragm, Pediatric Research, 82, (3) pp. 509-517. ISSN 0031-3998 (2017) [Refereed Article]

Copyright Statement

Copyright © 2017 International Pediatric Research Foundation, Inc.

DOI: doi:10.1038/pr.2017.99

Abstract

Background: Pregnant women at a high risk of preterm delivery receive glucocorticoids to accelerate fetal lung maturation and surfactant synthesis. However, the effect of antenatal steroids on the developing diaphragm remains unclear. We hypothesized that maternal betamethasone impairs the fetal diaphragm, and the magnitude of the detrimental effect increases with longer duration of exposure. We aimed to determine how different durations of fetal exposure to maternal betamethasone treatment influence the fetal diaphragm at the functional and molecular levels.

Methods: Date-mated merino ewes received intramuscular injections of saline (control) or two doses of betamethasone (5.7 mg) at an interval of 24 h commencing either 2 or 14 days before delivery. Preterm lambs were killed after cesarean delivery at 121-day gestational age. In vitro contractile measurements were performed on the right hemidiaphragm, whereas molecular/cellular analyses used the left costal diaphragm.

Results: Different durations of fetal exposure to maternal betamethasone had no consistent effect on the protein metabolic pathway, expression of glucocorticoid receptor and its target genes, cellular oxidative status, or contractile properties of the fetal lamb diaphragm.

Conclusion: These data suggest that the potential benefits of betamethasone exposure on preterm respiratory function are not compromised by impaired diaphragm function after low-dose maternal intramuscular glucocorticoid exposure.

Item Details

Item Type:Refereed Article
Keywords:diaphragm dysfunction, steriod exposure, fetal develoment
Research Division:Biomedical and Clinical Sciences
Research Group:Reproductive medicine
Research Field:Foetal development and medicine
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Song, Y (Dr Yong Song)
ID Code:135698
Year Published:2017
Web of Science® Times Cited:1
Deposited By:Menzies Institute for Medical Research
Deposited On:2019-11-08
Last Modified:2019-12-06
Downloads:0

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