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Osmotic sensitivity of Ca2+ and H+ transporters in corn roots: Effect on fluxes and their oscillations in the elongation region
Citation
Shabala, SN and Newman, IA, Osmotic sensitivity of Ca2+ and H+ transporters in corn roots: Effect on fluxes and their oscillations in the elongation region, The Journal of Membrane Biology, 161, (1) pp. 45-54. ISSN 0022-2631 (1998) [Refereed Article]
DOI: doi:10.1007/s002329900313
Abstract
Seedling roots of cam were treated with different concentrations of mannitol-containing solution for 1 to 1.5 hr, and net fluxes of Ca2+ and H+ were measured in the elongation region. H+ fluxes were much more sensitive to osmotic pressure than were Ca2+ fluxes. Oscillations of 7-min period in H+ flux, normally observed in the control, were almost fully suppressed at high osmotic concentrations. Net H+ flux was shifted from average efflux of 25 ± 3 nmol m-2 sec-1 to average influx of 10 ± 5 nmol m-2 sec-1 after the incubation in 100 mM mannitol. The larger the osmotic concentration, the larger was the H+ influx. This flux caused the unbuffered solution of pH 4.85 to change to pH 5.3 after mannitol application, It appears that the osmoticum suppresses oscillatory H+ extrusion at the plasma membrane. Discrete Fourier Transforms of the H+ flux data showed that, apart from suppression of the 7-min oscillations in H+ flux, mannitol also promoted the appearance of faster 2-min oscillations. Ca2+ influx slightly increased after mannitol treatment. In addition the 7-min oscillatory component of Ca2+ flux remained apparent thereby showing independence of H+ flux.
Item Details
Item Type: | Refereed Article |
---|---|
Research Division: | Physical Sciences |
Research Group: | Medical and biological physics |
Research Field: | Biological physics |
Objective Division: | Expanding Knowledge |
Objective Group: | Expanding knowledge |
Objective Field: | Expanding knowledge in the physical sciences |
UTAS Author: | Shabala, SN (Professor Sergey Shabala) |
UTAS Author: | Newman, IA (Dr Ian Newman) |
ID Code: | 13569 |
Year Published: | 1998 |
Web of Science® Times Cited: | 33 |
Deposited By: | Physics |
Deposited On: | 1998-08-01 |
Last Modified: | 2011-08-08 |
Downloads: | 0 |
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