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Stimulatory effect of plasma samples from fattening cattle on adipogenesis-related gene expression in preadipocyte cells

Citation

Watanabe, H and Chen, X and Shoji, N and Saito, R and Nakano, T and Saito, K and Sumiyoshi, K and Rose, MT and Okada, N and Watanabe, K and Aso, H, Stimulatory effect of plasma samples from fattening cattle on adipogenesis-related gene expression in preadipocyte cells, Animal Science Journal, 86, (7) pp. 698-706. ISSN 1344-3941 (2015) [Refereed Article]

Copyright Statement

Copyright 2014 Japanese Society of Animal Science

DOI: doi:10.1111/asj.12344

Abstract

It is desirable to produce beef with high levels of monounsaturated fatty acids (MUFA), as this is related to fat softness and palatability. However, the physiology of MUFA synthesis in bovine fat during the fattening process remains to be established. In this study, in order to elucidate the relationship between plasma components and the fatty acid composition of intramuscular fat, we investigated the effect of plasma obtained from fattening cattle on the messenger RNA (mRNA) expressions of the adipogenesis-related gene in a clonal bovine intramuscular preadipocyte line (BIP cells). The mRNA expressions of stearoyl-CoA desaturase, adipocyte Protein 2, peroxisome proliferator-activated receptor gamma and sterol regulatory element-binding protein 1 in BIP cells were significantly higher following treatment with those plasma samples collected from the cattle with the highest diaphragmatic unsaturated fatty acids to saturated fatty acids ratio (US/S). Furthermore, the concentration of nonesterified fatty acid (NEFA) in the plasma samples had an inverse correlation with carcass diaphragmatic US/S. These results indicate that cattle with a low ratio of US/S in fat may be discriminated from the population of fattening cattle before slaughter by measuring the effect of their plasma on gene expression in BIP cells as well as their plasma concentration and composition of NEFA.

Item Details

Item Type:Refereed Article
Keywords:adipogenesis, BIP cell, meat quality, NEFA, unsaturated fatty acid
Research Division:Agricultural and Veterinary Sciences
Research Group:Animal Production
Research Field:Animal Management
Objective Division:Animal Production and Animal Primary Products
Objective Group:Livestock Raising
Objective Field:Beef Cattle
UTAS Author:Rose, MT (Dr Michael Rose)
ID Code:135258
Year Published:2015
Deposited By:Office of the Tasmanian Institute of Agriculture
Deposited On:2019-10-08
Last Modified:2019-11-13
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