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Extracellular cyclophilin A possesses chemotaxic activity in cattle


Takanashi, S and Nochi, T and Abe, M and Itaya, N and Urakawa, M and Sato, K and Zhuang, T and Umemura, S and Hayashi, T and Kiku, Y and Kitazawa, H and Rose, MT and Watanabe, K and Aso, H, Extracellular cyclophilin A possesses chemotaxic activity in cattle, Veterinary Research, 46, (1) Article 80. ISSN 0928-4249 (2015) [Refereed Article]


Copyright Statement

2015 Takanashi et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License (

DOI: doi:10.1186/s13567-015-0212-1


Cyclophilin A (CyPA) was originally discovered in bovine thymocytes as a cytosolic binding protein of the immunosuppressive drug cyclosporine A. Recent studies have revealed that in mice and humans, CyPA is secreted from cells in injured or infected tissues and plays a role in recruiting inflammatory cells in those tissues. Here we found that in cattle abundant level of extracellular CyPA was observed in tissues with inflammation. To aid in investigating the role of extracellular CyPA in cattle, we generated recombinant bovine CyPA (rbCyPA) and tested its biological activity as an inflammatory mediator. When bovine peripheral blood cells were treated with rbCyPA in vitro, we observed that rbCyPA reacts with the membranous surface of granulocytes, monocytes and lymphocytes. Chemotaxis analysis showed that the granulocytes migrate toward rbCyPA and the migration is inhibited by pre-treatment with an anti-bovine CyPA antibody. These results indicate that, as for mice and humans, extracellular CyPA possesses chemotactic activity to recruit inflammatory cells (e.g., granulocytes) in cattle, and could thus be a potential therapeutic target for the treatment of inflammation.

Item Details

Item Type:Refereed Article
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Veterinary sciences
Research Field:Veterinary diagnosis and diagnostics
Objective Division:Animal Production and Animal Primary Products
Objective Group:Livestock raising
Objective Field:Dairy cattle
UTAS Author:Rose, MT (Dr Michael Rose)
ID Code:135257
Year Published:2015
Web of Science® Times Cited:9
Deposited By:Office of the Tasmanian Institute of Agriculture
Deposited On:2019-10-08
Last Modified:2019-11-15
Downloads:24 View Download Statistics

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