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Cyclophilin A is a new M cell marker of bovine intestinal epithelium

Citation

Hondo, T and Someya, S and Nagasawa, Y and Terada, S and Watanabe, H and Chen, X and Watanabe, K and Ohwada, S and Kitazawa, H and Rose, MT and Nochi, T and Aso, H, Cyclophilin A is a new M cell marker of bovine intestinal epithelium, Cell and Tissue Research, 364, (3) pp. 585-597. ISSN 0302-766X (2016) [Refereed Article]

Copyright Statement

Copyright 2016 Springer-Verlag Berlin Heidelberg

DOI: doi:10.1007/s00441-015-2342-1

Abstract

Microfold (M) cells in the follicle-associated epithelium (FAE) of Peyer’s patches contribute to the mucosal immune response by the transcytosis of microorganisms. The mechanism by which M cells take up microorganisms, and the functional proteins by which they do this, are not clear. In order to explore one such protein, we developed a 2H5-F3 monoclonal antibody (2H5-F3 mAb) through its binding to bovine M cells, and identified the antibody reactive molecule as cyclophilin A (Cyp-A). The localization patterns of Cyp-A were very similar to the localization pattern of cytokeratin (CK) 18-positive M cells. Cyp-A was identified at the luminal surface of CK18-positive M cells in bovine jejunal and ileal FAE. The membranous localization of Cyp-A in the bovine intestinal cell line (BIE cells) increased as cells differentiated toward M cells, as determined by flow cytometry analysis. Additionally, BIE cells released Cyp-A to the extracellular space and the differentiation of BIE cells to M cells increased the secretion of Cyp-A, as determined by western blotting. Accordingly, Cyp-A may be localized in M cells in the small intestinal epithelium of cattle. The rise of the membranous localization and secretion of Cyp-A by differentiation toward M cells indicates that Cyp-A has an important role in the function of M cells. While Cyp-A of the M cell membrane may contribute to the uptake of viruses with peptidyl-prolyl cis-trans isomerase activity, in the extracellular space Cyp-A may work as a chemokine and contribute to the distribution of immuno-competent cells.

Item Details

Item Type:Refereed Article
Keywords:M cell, follicle associated epithelium, Peyer’s patch, bovine intestinal epithelium, Cyclophilin A
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Animal physiology - cell
Objective Division:Animal Production and Animal Primary Products
Objective Group:Livestock raising
Objective Field:Beef cattle
UTAS Author:Rose, MT (Dr Michael Rose)
ID Code:135244
Year Published:2016
Web of Science® Times Cited:1
Deposited By:Office of the Tasmanian Institute of Agriculture
Deposited On:2019-10-08
Last Modified:2019-11-14
Downloads:0

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