eCite Digital Repository

Diversity and guaiacol production of Alicyclobacillus spp. from fruit juice and fruit-based beverages


Luong, TCV and Moir, C and Kaur, M and Frank, D and Bowman, JP and Bradbury, MI, Diversity and guaiacol production of Alicyclobacillus spp. from fruit juice and fruit-based beverages, International Journal of Food Microbiology, 311 Article 108314. ISSN 0168-1605 (2019) [Refereed Article]

Copyright Statement

Copyright 2019 Elsevier B.V.

DOI: doi:10.1016/j.ijfoodmicro.2019.108314


Alicyclobacillus acidoterrestris is an acido-thermophilic, spore-forming bacterial species that can spoil acidic fruit juice and fruit-based beverages. The metabolism of taint compounds by this bacterial species has led to its status as a targeted microorganism in the fruit juice industry. This study aims to assess the genetic diversity of Alicyclobacillus spp. including A. acidoterrestris and its correlation to spoilage taint metabolism. Alicyclobacillus cultures, which were previously isolated from a wide range of domestic and international products including fruit juice, fruit drinks and fruit juice concentrates, were subjected to DNA fingerprint analysis by using randomly amplified polymorphic DNA (RAPD) - polymerase chain reaction. Isolates were classified on the basis of their RAPD profile and the results were used to select representative strains to undergo taint production assessment. The taint guaiacol produced by Alicyclobacillus spp. was measured by headspace gas chromatography and mass spectrometry. From produced RAPD profiles, two genotypic groups and two sub-groups were identified. The groups were independent of product types and geographical origins. A significant number of isolates were clustered in genotypic group I, including A. acidoterrestris ATCC 49025. These isolates produced significant levels of guaiacol, 8.7 mg/L on average. A smaller number of isolates was found in genotypic group II including A. acidocaldarius and they produced no guaiacol. Primer F-64 was useful to identify Alicyclobacillus at the species level, and permitted rapid identification of strains producing fruit juice taint compounds such as guaiacol.

Item Details

Item Type:Refereed Article
Keywords:bacterial spores, spoilage microorganisms, acidic food stability, guaiacol RAPD-PCR, fruit juice spoilage, Alicyclobacillus
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Food sciences
Research Field:Food packaging, preservation and processing
Objective Division:Manufacturing
Objective Group:Processed food products and beverages (excl. dairy products)
Objective Field:Processed fruit and vegetable products (incl. juices)
UTAS Author:Luong, TCV (Mr Van Luong)
UTAS Author:Kaur, M (Miss Maanpreet Kaur)
UTAS Author:Bowman, JP (Associate Professor John Bowman)
ID Code:135127
Year Published:2019
Web of Science® Times Cited:5
Deposited By:TIA - Research Institute
Deposited On:2019-10-01
Last Modified:2020-03-31

Repository Staff Only: item control page