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Antibiotic resistance, putative virulence factors and curli fimbrination among Cronobacter species

Citation

Odeyemi, OA and Abdullah Sani, N, Antibiotic resistance, putative virulence factors and curli fimbrination among Cronobacter species, Microbial Pathogenesis, 136 Article 103665. ISSN 0882-4010 (2019) [Refereed Article]

Copyright Statement

Copyright 2019 Elsevier Ltd.

DOI: doi:10.1016/j.micpath.2019.103665

Abstract

This study aimed to investigate antibiotic resistance and putative virulence factors among Cronobacter sakazakii isolated from powdered infant formula and other sources. The following 9 cultures (CR1-9) were collected from our culture collection: C. sakazakii and 3 Cronobacter species: C. sakazakii ATCC® 29544™, C. muytjensii ATCC® 51329™, C. turicensis E866 were used in this study. Isolates were subjected to antibiotic susceptibility and the following virulence factors (protease, DNase, haemolysin, gelatinase, motility and biofilm formation) using phenotypic methods. All the bacteria were able to form biofilm on agar at 37 °C and were resistant to ampicillin, erythromycin, fosfomycin and sulphamethoxazole. It was observed from this study that tested strains formed weak and strong biofilm with violet dry and rough (rdar), brown dry and rough (bdar), red mucoid and smooth (rmas) colony morphotypes on Congo red agar. Rdar expresses curli and fimbriae, while bdar expresses curli. Both biofilm colony morphotypes are commonly found in Enterobacteriaceae including Salmonella species. This study also reveals a new colony morphotypes in Cronobacter species. Conclusively, there was correlation between putative virulence factors and antibiotic resistance among the tested bacteria. Further study on virulence and antibiotic resistance genes is hereby encouraged.

Item Details

Item Type:Refereed Article
Keywords:virulence factor, biofilm formation, antibiotic resistance, Cronobacter sakazakii
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Microbiology not elsewhere classified
Objective Division:Manufacturing
Objective Group:Dairy Products
Objective Field:Dairy Products not elsewhere classified
UTAS Author:Odeyemi, OA (Dr Olumide Odeyemi)
ID Code:135107
Year Published:2019
Web of Science® Times Cited:1
Deposited By:Ecology and Biodiversity
Deposited On:2019-09-30
Last Modified:2020-01-07
Downloads:0

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