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The RNA-binding protein HuR (Elavl1) is essential for the B cell antibody response
Citation
Diaz-Munoz, MD and Bell, SE and Fairfax, F and Monzon-Casanova, E and Cunningham, AF and Gonzalez-Porta, M and Andrews, SR and Bunik, VI and Zarnack, K and Curk, T and Heggermont, WA and Heymans, S and Gibson, GE and Kontoyiannis, DL and Ule, J and Turner, M, The RNA-binding protein HuR (Elavl1) is essential for the B cell antibody response, Nature Immunology, 16, (4) pp. 415-425. ISSN 1529-2908 (2015) [Refereed Article]
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Copyright Statement
Copyright 2015 the authors. Post-prints are subject to Springer Nature re-use terms
DOI: doi:10.1038/ni.3115
Abstract
Post-transcriptional regulation of mRNA by the RNA-binding protein HuR (encoded by Elavl1) is required in B cells for the germinal center reaction and for the production of class-switched antibodies in response to thymus-independent antigens. Transcriptome-wide examination of RNA isoforms and their abundance and translation in HuR-deficient B cells, together with direct measurements of HuR-RNA interactions, revealed that HuR-dependent splicing of mRNA affected hundreds of transcripts, including that encoding dihydrolipoamide S-succinyltransferase (Dlst), a subunit of the 2-oxoglutarate dehydrogenase (α-KGDH) complex. In the absence of HuR, defective mitochondrial metabolism resulted in large amounts of reactive oxygen species and B cell death. Our study shows how post-transcriptional processes control the balance of energy metabolism required for the proliferation and differentiation of B cells.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Biological Sciences |
| Research Group: | Biochemistry and Cell Biology |
| Research Field: | Cell Development, Proliferation and Death |
| Objective Division: | Health |
| Objective Group: | Clinical Health (Organs, Diseases and Abnormal Conditions) |
| Objective Field: | Immune System and Allergy |
| UTAS Author: | Fairfax, F (Dr Kirsten Fairfax) |
| ID Code: | 134803 |
| Year Published: | 2015 |
| Web of Science® Times Cited: | 56 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2019-09-05 |
| Last Modified: | 2019-10-16 |
| Downloads: | 3 View Download Statistics |
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