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The RNA-binding protein HuR (Elavl1) is essential for the B cell antibody response

Citation

Diaz-Munoz, MD and Bell, SE and Fairfax, F and Monzon-Casanova, E and Cunningham, AF and Gonzalez-Porta, M and Andrews, SR and Bunik, VI and Zarnack, K and Curk, T and Heggermont, WA and Heymans, S and Gibson, GE and Kontoyiannis, DL and Ule, J and Turner, M, The RNA-binding protein HuR (Elavl1) is essential for the B cell antibody response, Nature Immunology, 16, (4) pp. 415-425. ISSN 1529-2908 (2015) [Refereed Article]


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Copyright 2015 the authors. Post-prints are subject to Springer Nature re-use terms

DOI: doi:10.1038/ni.3115

Abstract

Post-transcriptional regulation of mRNA by the RNA-binding protein HuR (encoded by Elavl1) is required in B cells for the germinal center reaction and for the production of class-switched antibodies in response to thymus-independent antigens. Transcriptome-wide examination of RNA isoforms and their abundance and translation in HuR-deficient B cells, together with direct measurements of HuR-RNA interactions, revealed that HuR-dependent splicing of mRNA affected hundreds of transcripts, including that encoding dihydrolipoamide S-succinyltransferase (Dlst), a subunit of the 2-oxoglutarate dehydrogenase (α-KGDH) complex. In the absence of HuR, defective mitochondrial metabolism resulted in large amounts of reactive oxygen species and B cell death. Our study shows how post-transcriptional processes control the balance of energy metabolism required for the proliferation and differentiation of B cells.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Biochemistry and Cell Biology
Research Field:Cell Development, Proliferation and Death
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Immune System and Allergy
UTAS Author:Fairfax, F (Dr Kirsten Fairfax)
ID Code:134803
Year Published:2015
Web of Science® Times Cited:56
Deposited By:Menzies Institute for Medical Research
Deposited On:2019-09-05
Last Modified:2019-10-16
Downloads:3 View Download Statistics

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