134797 - NF&%23954;B1 is essential to prevent the development of multiorgan.pdf (4.62 MB)
NFκB1 is essential to prevent the development of multiorgan autoimmunity by limiting IL-6 production in follicular B cells
journal contribution
posted on 2023-05-20, 06:55 authored by de Valle, E, Grigoriadis, G, O'Reilly, LA, Willis, SN, Maxwell, MJ, Corcoran, LM, Tsantikos, E, Cornish, JK, Kirsten FairfaxKirsten Fairfax, Vasanthakumar, A, Febbraio, MA, Hibbs, ML, Pellegrini, M, Banerjee, A, Hodgkin, PD, Kallies, A, Mackay, F, Strasser, A, Gerondakis, S, Gugasyan, RWe examined the role of NFκB1 in the homeostasis and function of peripheral follicular (Fo) B cells. Aging mice lacking NFκB1 (Nfκb1(-/-) develop lymphoproliferative and multiorgan autoimmune disease attributed in large part to the deregulated activity of Nfκb1(-/-) Fo B cells that produce excessive levels of the proinflammatory cytokine interleukin 6 (IL-6). Despite enhanced germinal center (GC) B cell differentiation, the formation of GC structures was severely disrupted in the Nfκb1(-/-) mice. Bone marrow chimeric mice revealed that the Fo B cell-intrinsic loss of NFκB1 led to the spontaneous generation of GC B cells. This was primarily the result of an increase in IL-6 levels, which promotes the differentiation of Fo helper CD4(+)T cells and acts in an autocrine manner to reduce antigen receptor and toll-like receptor activation thresholds in a population of proliferating IgM(+)Nfκb1(-/-) Fo B cells. We demonstrate that p50-NFκB1 represses Il-6 transcription in Fo B cells, with the loss of NFκB1 also resulting in the uncontrolled RELA-driven transcription of Il-6. Collectively, our findings identify a previously unrecognized role for NFκB1 in preventing multiorgan autoimmunity through its negative regulation of Il-6 gene expression in Fo B cells.
History
Publication title
The Journal of Experimental MedicineVolume
213Issue
4Pagination
621-641ISSN
0022-1007Department/School
Menzies Institute for Medical ResearchPublisher
Rockefeller Univ PressPlace of publication
1114 First Ave, 4Th Fl, New York, USA, Ny, 10021Rights statement
© 2016 de Valle et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial– Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).Repository Status
- Open