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Comparative gonad histology and semen quality of normal (XY) and neo-males (XX) of Atlantic salmon (Salmo salar)

Citation

de Castro, PL and Patil, JG, Comparative gonad histology and semen quality of normal (XY) and neo-males (XX) of Atlantic salmon (Salmo salar), Aquaculture Research, 50, (11) pp. 3171-3180. ISSN 1355-557X (2019) [Refereed Article]

Copyright Statement

Copyright 2019 John Wiley & Sons Ltd.

DOI: doi:10.1111/are.14271

Abstract

With an overarching objective of improving the hatchery production of Atlantic salmon (Salmo salar) all‐female progeny, this study comparatively evaluated the reproductive parameters between normal (genotype XY) and neo‐males (genotype XX). Four normal (XY) and seven neo‐ (XX) males, from the same brood stock, distinguished by their ability to or lack of expressing semen, respectively, were comparatively evaluated. The left testicular lobe was used for histomorphometric analyses, while the right for semen collection and sperm quality analyses. Histomorphometric observations revealed that neo‐male testes are irregularly shaped, and have poorly formed seminiferous ducts, higher proportions of interstitial tissue and lower gonadosomatic index (p<.05). In addition, hypertrophied and cyst forming Sertoli cells were found in these individuals which collectively appear to form a physical barrier, precluding the semen collection by standard stripping techniques and reducing sperm quality. Particularly, semen motility (80.692.4% and 57.236.5% for XY and XX respectively) and duration of motility (99.3128.03s and 66.8423.83s for XY and XX respectively) of neo‐males were most compromised (p<.05). Interestingly, the TUNEL assay indicated no signs of apoptotic tissue suggesting that the histological differences may relate to delayed physiological/sexual maturity of neo‐males.

Item Details

Item Type:Refereed Article
Keywords:Atlantic salmon, neomale, CASA, sperm, computer-assisted semen analysis, fish reproduction, gonadal development, gonadal histology of fish, monosex in aquaculture
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Aquaculture fin fish (excl. tuna)
UTAS Author:Patil, JG (Dr Jawahar Patil)
ID Code:134721
Year Published:2019
Web of Science® Times Cited:2
Deposited By:Ecology and Biodiversity
Deposited On:2019-08-29
Last Modified:2020-01-07
Downloads:0

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