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Synbiotic supplementation containing whole plant sugar cane Fibre and probiotic spores potentiates protective synergistic effects in mouse model of IBD

Citation

Shinde, T and Perera, AP and Vemuri, R and Gondalia, SV and Karpe, AV and Beale, DJ and Shastri, M and Southam, BT and Eri, R and Stanley, R, Synbiotic supplementation containing whole plant sugar cane Fibre and probiotic spores potentiates protective synergistic effects in mouse model of IBD, Nutrients, 11, (4) pp. 1-24. ISSN 2072-6643 (2019) [Refereed Article]


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Copyright 2019 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3390/nu11040818

Abstract

Inflammatory bowel diseases (IBD) are a chronic inflammatory disorders with increasing global incidence. Synbiotic, which is a two-point approach carrying probiotic and prebiotic components in mitigating inflammation in IBD, is thought to be a pragmatic approach owing to the synergistic outcomes. In this study, the impacts of dietary supplementation with probiotic Bacillus coagulansMTCC5856 spores (B. coagulans) and prebiotic whole plant sugar cane fibre (PSCF) was assessed using a murine model of IBD. Eight-week-old C57BL/6 mice were fed a normal chow diet supplemented with either B. coagulans, PSCF or its synbiotic combination. After seven days of supplementation, colitis was induced with dextran sulfate sodium (DSS) in drinking water for seven days during the continuation of the supplemented diets. Synbiotic supplementation ameliorated disease activity index and histological score (−72%, 7.38, respectively), more effectively than either B. coagulans (−47%, 10.1) and PSCF (−53%, 13.0) alone. Synbiotic supplementation also significantly (p < 0.0001) prevented the expression of tight junction proteins and modulated the altered serum IL-1β (−40%), IL-10 (+26%), and C-reactive protein (CRP) (−39%) levels. Synbiotic supplementations also raised the short-chain fatty acids (SCFA) profile more extensively compared to the unsupplemented DSS-control. The synbiotic health outcome effect of the probiotic and prebiotic combinations may be associated with a synergistic direct immune-regulating efficacy of the components, their ability to protect epithelial integrity, stimulation of probiotic spores by the prebiotic fibre, and/or with stimulation of greater levels of fermentation of fibres releasing SCFAs that mediate the reduction in colonic inflammation. Our model findings suggest synbiotic supplementation should be tested in clinical trials.

Item Details

Item Type:Refereed Article
Keywords:synbiotic, prebiotic, probiotic, IBD, &lt;i&gt;Bacillus&lt;/i&gt; spores,dietary fibre, sugar cane fibre, ulcerative colitis
Research Division:Medical and Health Sciences
Research Group:Clinical Sciences
Research Field:Gastroenterology and Hepatology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Digestive System Disorders
UTAS Author:Shinde, T (Ms Tanvi Shinde)
UTAS Author:Perera, AP (Mrs Agampodi Perera)
UTAS Author:Vemuri, R (Mr Ravichandra Vemuri)
UTAS Author:Shastri, M (Mr Madhur Shastri)
UTAS Author:Southam, BT (Mr Benjamin Southam)
UTAS Author:Eri, R (Associate Professor Raj Eri)
UTAS Author:Stanley, R (Professor Roger Stanley)
ID Code:134585
Year Published:2019
Deposited By:Health Sciences
Deposited On:2019-08-21
Last Modified:2019-09-05
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