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Synbiotic supplementation containing whole plant sugar cane Fibre and probiotic spores potentiates protective synergistic effects in mouse model of IBD
Citation
Shinde, T and Perera, AP and Vemuri, R and Gondalia, SV and Karpe, AV and Beale, DJ and Shastri, M and Southam, BT and Eri, R and Stanley, R, Synbiotic supplementation containing whole plant sugar cane Fibre and probiotic spores potentiates protective synergistic effects in mouse model of IBD, Nutrients, 11, (4) pp. 1-24. ISSN 2072-6643 (2019) [Refereed Article]
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Copyright Statement
Copyright 2019 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Abstract
Inflammatory bowel diseases (IBD) are a chronic inflammatory disorders with increasing
global incidence. Synbiotic, which is a two-point approach carrying probiotic and prebiotic
components in mitigating inflammation in IBD, is thought to be a pragmatic approach owing to the
synergistic outcomes. In this study, the impacts of dietary supplementation with probiotic Bacillus
coagulansMTCC5856 spores (B. coagulans) and prebiotic whole plant sugar cane fibre (PSCF) was
assessed using a murine model of IBD. Eight-week-old C57BL/6 mice were fed a normal chow
diet supplemented with either B. coagulans, PSCF or its synbiotic combination. After seven days of
supplementation, colitis was induced with dextran sulfate sodium (DSS) in drinking water for seven
days during the continuation of the supplemented diets. Synbiotic supplementation ameliorated
disease activity index and histological score (−72%, 7.38, respectively), more effectively than either
B. coagulans (−47%, 10.1) and PSCF (−53%, 13.0) alone. Synbiotic supplementation also significantly
(p < 0.0001) prevented the expression of tight junction proteins and modulated the altered serum IL-1β
(−40%), IL-10 (+26%), and C-reactive protein (CRP) (−39%) levels. Synbiotic supplementations also
raised the short-chain fatty acids (SCFA) profile more extensively compared to the unsupplemented
DSS-control. The synbiotic health outcome effect of the probiotic and prebiotic combinations may
be associated with a synergistic direct immune-regulating efficacy of the components, their ability
to protect epithelial integrity, stimulation of probiotic spores by the prebiotic fibre, and/or with
stimulation of greater levels of fermentation of fibres releasing SCFAs that mediate the reduction in
colonic inflammation. Our model findings suggest synbiotic supplementation should be tested in
clinical trials.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | synbiotic, prebiotic, probiotic, IBD, <i>Bacillus</i> spores,dietary fibre, sugar cane fibre, ulcerative colitis |
| Research Division: | Medical and Health Sciences |
| Research Group: | Clinical Sciences |
| Research Field: | Gastroenterology and Hepatology |
| Objective Division: | Health |
| Objective Group: | Clinical Health (Organs, Diseases and Abnormal Conditions) |
| Objective Field: | Digestive System Disorders |
| UTAS Author: | Shinde, T (Ms Tanvi Shinde) |
| UTAS Author: | Perera, AP (Mrs Agampodi Perera) |
| UTAS Author: | Vemuri, R (Mr Ravichandra Vemuri) |
| UTAS Author: | Shastri, M (Mr Madhur Shastri) |
| UTAS Author: | Southam, BT (Mr Benjamin Southam) |
| UTAS Author: | Eri, R (Associate Professor Raj Eri) |
| UTAS Author: | Stanley, R (Professor Roger Stanley) |
| ID Code: | 134585 |
| Year Published: | 2019 |
| Web of Science® Times Cited: | 1 |
| Deposited By: | Health Sciences |
| Deposited On: | 2019-08-21 |
| Last Modified: | 2019-09-05 |
| Downloads: | 4 View Download Statistics |
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