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Pharmaceutical and preclinical evaluation of Advax adjuvant as a dose-sparing strategy for ant venom immunotherapy

Citation

Wanandy, T and Honda-Okubo, Y and Davies, NW and Rose, HE and Heddle, RJ and Brown, SGA and Woodman, RJ and Petrovsky, N and Wiese, MD, Pharmaceutical and preclinical evaluation of Advax adjuvant as a dose-sparing strategy for ant venom immunotherapy, Journal of Pharmaceutical and Biomedical Analysis, 172 pp. 1-8. ISSN 0731-7085 (2019) [Refereed Article]

Copyright Statement

© 2019 Elsevier B.V. All rights reserved.

DOI: doi:10.1016/j.jpba.2019.04.017

Abstract

A major challenge in broader clinical application of Jack Jumper ant venom immunotherapy (JJA VIT) is the scarcity of ant venom which needs to be manually harvested from wild ants. Adjuvants are commonly used for antigen sparing in other vaccines, and thereby could potentially have major benefits to extend JJA supplies if they were to similarly enhance JJA VIT immunogenicity. The purpose of this study was to evaluate the physicochemical and microbiological stability and murine immunogenicity of low-dose JJA VIT formulated with a novel polysaccharide adjuvant referred to as delta inulin or Advax™. Jack Jumper ant venom (JJAV) protein stability was assessed by UPLC-UV, SDS-PAGE, SDS-PAGE immunoblot, and ELISA inhibition. Diffraction light scattering was used to assess particle size distribution of Advax; pH and benzyl alcohol quantification by UPLC-UV were used to assess the physicochemical stability of JJAV diluent, and endotoxin content and preservative efficacy test was used to investigate the microbiological properties of the adjuvanted VIT formulation. To assess the effect of adjuvant on JJA venom immunogenicity, mice were immunised four times with JJAV alone or formulated with Advax adjuvant. JJA VIT formulated with Advax was found to be physicochemically and microbiologically stable for at least 2 days when stored at 4 and 25 °C with a trend for an increase in allergenic potency observed beyond 2 days of storage. Low-dose JJAV formulated with Advax adjuvant induced significantly higher JJAV-specific IgG than a 5-fold higher dose of JJAV alone, consistent with a powerful allergen-sparing effect. The pharmaceutical data provides important guidance on the formulation, storage and use of JJA VIT formulated with Advax adjuvant, with the murine immunogenicity studies providing a strong rationale for a planned clinical trial to test the ability of Advax adjuvant to achieve 4-fold JJAV dose sparing in JJA-allergic human patients.

Item Details

Item Type:Refereed Article
Keywords:adjuvants, Advax, allergy, delta inulin, venom immunotherapy
Research Division:Medical and Health Sciences
Research Group:Pharmacology and Pharmaceutical Sciences
Research Field:Toxicology (incl. Clinical Toxicology)
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Immune System and Allergy
UTAS Author:Wanandy, T (Mr Troy Wanandy)
UTAS Author:Davies, NW (Associate Professor Noel Davies)
UTAS Author:Brown, SGA (Professor Simon Brown)
ID Code:134582
Year Published:2019
Web of Science® Times Cited:1
Deposited By:Pharmacy
Deposited On:2019-08-21
Last Modified:2019-09-12
Downloads:1 View Download Statistics

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