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Limitations of a hemolytic plaque assay for IgG-anti-IgG rheumatoid factor-producing cells


Venn, AJ and Dresser, DW, Limitations of a hemolytic plaque assay for IgG-anti-IgG rheumatoid factor-producing cells, Journal of Immunological Methods, 102, (2) pp. 195-204. ISSN 0022-1759 (1987) [Refereed Article]

DOI: doi:10.1016/0022-1759(87)90077-9


An attempt has been made to develop a hemolytic plaque assay capable of detecting homophile IgG rheumatoid factor (RF)-producing cells. Anti-immunoglobulin allotype-developing reagents were used to distinguish between target and effector IgG. The hemolytic assay has been used to demonstrate an apparently high level of homophile IgM and IgG RF-producing cells in the spleens and lymph nodes of mice stimulated by LPS. However, it appears that a large proportion of the plaques obtained in these assays are due to an artefact resulting from cross-linking of target and effector molecules by the developing reagents. In the case of IgM RF the artefact depends on the presence of a small contamination of the target IgG by IgM, allowing cross-linking of target and effector IgM by the anti-mu-specific developing reagent. With the IgG RF, cross-reactivity of the rabbit anti-Ighb allotype-developing serum for the 'wrong' (Igha) allotype, normally undetectable, becomes sufficient to be biologically relevant when the developing antibody is complexed by being bound to its target (Ighb) allotype. Nevertheless anti-allotype reagents may afford an accurate means of detecting homophile IgG RF producing cells using other assay systems.

Item Details

Item Type:Refereed Article
Keywords:rheumatoid factor, IgM rheumatoid factor plaque-forming cell, IgG rheumatoiid factor plaque-forming cell, allotype
Research Division:Biomedical and Clinical Sciences
Research Group:Immunology
Research Field:Cellular immunology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Venn, AJ (Professor Alison Venn)
ID Code:134348
Year Published:1987
Web of Science® Times Cited:4
Deposited By:Menzies Institute for Medical Research
Deposited On:2019-08-08
Last Modified:2019-08-08

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