Bindal, P and Gnanasegaran, N and Bindal, U and Haque, N and Ramasamy, TS and Chai, WL and Kasim, NHA, Angiogenic effect of platelet-rich concentrates on dental pulp stem cells in inflamed microenvironment, Clinical Oral Investigations, 23, (10) pp. 3821-3831. ISSN 1432-6981 (2019) [Refereed Article]
Copyright Springer-Verlag GmbH Germany, part of Springer Nature 2019
Objective: In this study, we aimed to determine the suitable concentrations of human platelet lysate (HPL) and platelet-rich plasma (PRP) for maintaining the in vitro proliferative and angiogenic potential of inflamed dental pulp stem cells.
Materials and methods: (LPS)-induced inflamed dental pulp-derived stem cells (iDPSCs) were treated with different concentrations of HPL and PRP (10% and 20%) followed by determination of viability using Alamar Blue assay. Expression of angiogenesis-, adhesion-, and inflammation-regulating genes was also analyzed using RT-qPCR array. Furthermore, expression of growth factors at protein level in the cell culture microenvironment was measured using multiplex assay.
Results: Viability of iDPSCs was significantly (p < 0.05) higher in 20% HPL-supplemented media compared to iDPSCs. Expression of 10 out of 12 selected angiogenic genes, four out of seven adhesion molecules, and seven out of nine cytokineproducing genes were significantly (p < 0.05) higher in cells maintained in 20% HPL-supplemented media compared to that in FBS-supplemented media. Furthermore, expression of all the selected growth factors was significantly higher (p < 0.05) in the supernatants from 20% HPL media at 12 and 24 h post-incubation.
Conclusion: This study suggests that 20% HPL could be optimum to stimulate angiogenesis-related factors in iDPSCs while maintaining their viability.
Clinical relevance: This data may suggest the potential use of 20% HPL for expanding DPSCs scheduled for clinical trials for regenerative therapies including dental pulp regeneration.
|Item Type:||Refereed Article|
|Keywords:||cytokines, growth factors, platelet lysate, platelet-rich plasma, viability|
|Research Division:||Biomedical and Clinical Sciences|
|Research Field:||Dental therapeutics, pharmacology and toxicology|
|Objective Division:||Expanding Knowledge|
|Objective Group:||Expanding knowledge|
|Objective Field:||Expanding knowledge in the health sciences|
|UTAS Author:||Bindal, P (Mrs Priyadarshni Bindal)|
|Web of Science® Times Cited:||9|
|Deposited By:||Health Sciences|
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