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C9orf72 expansion disrupts ATM-mediated chromosomal break repair


Walker, C and Herranz-Martin, S and Karyka, E and Liao, C and Lewis, K and Elsayed, W and Lukashchuk, V and Chiang, S-C and Ray, S and Mulcahy, PJ and Jurga, M and Tsagakis, I and Iannitti, T and Chandran, J and Coldicott, I and De Vos, KJ and Hassan, MK and Higginbottom, A and Shaw, PJ and Hautbergue, GM and Azzouz, M and El-Khamisy, SF, C9orf72 expansion disrupts ATM-mediated chromosomal break repair, Nature Neuroscience, 20, (9) pp. 1225-1235. ISSN 1097-6256 (2017) [Refereed Article]

DOI: doi:10.1038/nn.4604


Hexanucleotide repeat expansions represent the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia, though the mechanisms by which such expansions cause neurodegeneration are poorly understood. We report elevated levels of DNA-RNA hybrids (R-loops) and double strand breaks in rat neurons, human cells and C9orf72 ALS patient spinal cord tissues. Accumulation of endogenous DNA damage is concomitant with defective ATM-mediated DNA repair signaling and accumulation of protein-linked DNA breaks. We reveal that defective ATM-mediated DNA repair is a consequence of P62 accumulation, which impairs H2A ubiquitylation and perturbs ATM signaling. Virus-mediated expression of C9orf72-related RNA and dipeptide repeats in the mouse central nervous system increases double strand breaks and ATM defects and triggers neurodegeneration. These findings identify R-loops, double strand breaks and defective ATM-mediated repair as pathological consequences of C9orf72 expansions and suggest that C9orf72-linked neurodegeneration is driven at least partly by genomic instability.

Item Details

Item Type:Refereed Article
Research Division:Biomedical and Clinical Sciences
Research Group:Neurosciences
Research Field:Neurology and neuromuscular diseases
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Lewis, K (Dr Katherine Lewis)
ID Code:133966
Year Published:2017
Web of Science® Times Cited:101
Deposited By:Menzies Institute for Medical Research
Deposited On:2019-07-17
Last Modified:2019-07-17

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