Hisada, Y and Grover, S and Maqsood, A and Houston, R and Ay, C and Nouboussie, D and Cooley, B and Wallen, H and Key, N and Thalin, C and Farkas, A and Tenekedjiev, K and Kolev, K and Mackman, N, Role of neutrophils and neutrophil extracellular traps in mice bearing human pancreatic tumors, Research and Practice in Thrombosis and Haemostasis, 3, (51) Article OC30.4. ISSN 2475-0379 (2019) [Contribution to Refereed Journal]
Aims : To determine the contribution of neutrophils and NETs to venous thrombosis in mice bearing human pancreatic tumors.
Methods : Nude mice bearing human pancreatic BxPc- 3 tumors were used. Thrombosis was induced by inferior vena cava stasis.
Results : We found that tumor- bearing mice had increased circulating neutrophil counts and levels of granulocyte- colony stimulating factor, neutrophil elastase, H3Cit and cell- free DNA compared with controls. In addition, thrombi from tumor- bearing mice contained increased levels of the neutrophil marker Ly6G, as well as higher levels of H3Cit and extracellular DNA. Thrombi from tumor- bearing mice also had thinner fibrin fibers consistent with increased thrombin generation. Importantly, neutrophil depletion and administration of DNase I reduced thrombus size in tumor- bearing but not in control mice.
Conclusions : Our data indicate that neutrophils and NETs contribute to venous thrombosis in a mouse model of pancreatic cancer. Targeting this pathway may prevent VTE in pancreatic cancer patients without increasing bleeding.
|Item Type:||Contribution to Refereed Journal|
|Keywords:||neutrophils, NET, venous thrombosis, mice, pancreatic tumors|
|Research Division:||Information and Computing Sciences|
|Research Group:||Artificial Intelligence and Image Processing|
|Research Field:||Simulation and Modelling|
|Objective Division:||Expanding Knowledge|
|Objective Group:||Expanding Knowledge|
|Objective Field:||Expanding Knowledge in the Medical and Health Sciences|
|UTAS Author:||Tenekedjiev, K (Professor Kiril Tenekedjiev)|
|Deposited By:||NC Maritime Engineering and Hydrodynamics|
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