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Recellularization of auricular cartilage via elastase-generated channels


Lehmann, J and Nurnberger, S and Narcisi, R and Stok, KS and van der Eerden, BCJ and Koevoet, WJLM and Kops, N and ten Berge, D and van Osch, GJ, Recellularization of auricular cartilage via elastase-generated channels, Biofabrication, 11, (3) pp. 035012. ISSN 1758-5090 (2019) [Refereed Article]

Copyright Statement

2019 IOP Publishing Ltd

DOI: doi:10.1088/1758-5090/ab1436


Decellularized tissue matrices are promising substrates for tissue generation by stem cells to replace poorly regenerating tissues such as cartilage. However, the dense matrix of decellularized cartilage impedes colonisation by stem cells. Here, we show that digestion of elastin fibre bundles traversing auricular cartilage creates channels through which cells can migrate into the matrix. Human chondrocytes and bone marrow-derived mesenchymal stromal cells efficiently colonise elastin-treated scaffolds through these channels, restoring a glycosaminoglycan-rich matrix and improving mechanical properties while maintaining size and shape of the restored tissue. The scaffolds are also rapidly colonised by endogenous cartilage-forming cells in a subcutaneously implanted osteochondral biopsy model. Creating channels for cells in tissue matrices may be a broadly applicable strategy for recellularization and restoration of tissue function.

Item Details

Item Type:Refereed Article
Keywords:cartilage tissue engineering, recellularization, auricular, elastin, osteochondral defect
Research Division:Engineering
Research Group:Biomedical engineering
Research Field:Biomechanical engineering
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in engineering
UTAS Author:Stok, KS (Dr Kathryn Stok)
ID Code:133664
Year Published:2019
Web of Science® Times Cited:3
Deposited By:Menzies Institute for Medical Research
Deposited On:2019-07-05
Last Modified:2019-08-30

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