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Developing staining protocols for visualization of tissue-engineering scaffolds using micro computed tomography in native wet state

Citation

Kuo, W and Nimeskern, L and Martinez Avila, H and Hofmann, S and Freedman, J and Grinstaff, MW and Muller, R and Stok, KS, Developing staining protocols for visualization of tissue-engineering scaffolds using micro computed tomography in native wet state, Biomedizinische Technik, 58, (Suppl. 1) ISSN 0013-5585 (2013) [Contribution to Refereed Journal]


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DOI: doi:10.1515/bmt-2013-4273

Abstract

BNC-alginate and silk fibroin tissue-engineering scaffolds were stained with X-ray contrast agents in order to visualize internal microstructure in the native wet state with microcomputed tomography. A successful protocol employing amphiphilic contrast agents (CAs) dissolved in a water-based staining solution was used. The CAs were then fixed to the scaffold by neutralizing their charged functional groups, increasing their hydrophobicity and retention on the scaffold surface in water. While some unresolved issues concerning homogeneous staining and strength of contrast remain, these first successes constitute an important milestone by identifying good contrast agent candidates and staining protocols for longitudinal monitoring of tissue-engineering studies.

Item Details

Item Type:Contribution to Refereed Journal
Keywords:contrast agent, silk fibroin, bacterial nanocellu-lose, alginate
Research Division:Engineering
Research Group:Biomedical engineering
Research Field:Biomechanical engineering
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in engineering
UTAS Author:Stok, KS (Dr Kathryn Stok)
ID Code:133653
Year Published:2013
Deposited By:Menzies Institute for Medical Research
Deposited On:2019-07-05
Last Modified:2019-07-05
Downloads:0

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