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Developing staining protocols for visualization of tissue-engineering scaffolds using micro computed tomography in native wet state
journal contribution
posted on 2023-05-22, 03:44 authored by Kuo, W, Nimeskern, L, Martinez Avila, H, Hofmann, S, Freedman, J, Grinstaff, MW, Muller, R, Kathryn StokBNC-alginate and silk fibroin tissue-engineering scaffolds were stained with X-ray contrast agents in order to visualize internal microstructure in the native wet state with microcomputed tomography. A successful protocol employing amphiphilic contrast agents (CAs) dissolved in a water-based staining solution was used. The CAs were then fixed to the scaffold by neutralizing their charged functional groups, increasing their hydrophobicity and retention on the scaffold surface in water. While some unresolved issues concerning homogeneous staining and strength of contrast remain, these first successes constitute an important milestone by identifying good contrast agent candidates and staining protocols for longitudinal monitoring of tissue-engineering studies.
History
Publication title
Biomedizinische TechnikVolume
58Issue
Suppl. 1ISSN
0013-5585Department/School
Menzies Institute for Medical ResearchPublisher
Walter de Gruyter GmbHPlace of publication
Markgrafenstrasse 11, Berlin, Germany, D-10969Repository Status
- Restricted