133649 - Generation of vestibular tissue-like organoids from human pluripotent stem cells using the rotary cell culture system.pdf (5.45 MB)
Generation of vestibular tissue-like organoids from human pluripotent stem cells using the rotary cell culture system
journal contribution
posted on 2023-05-20, 05:16 authored by Mattei, C, Lim, R, Drury, H, Nasr, B, Li, Z, Tadros, MA, D'Abaco, GM, Kathryn Stok, Nayagam, BA, Dottori, MHair cells are specialized mechanosensitive cells responsible for mediating balance and hearing within the inner ear. In mammals, hair cells are limited in number and do not regenerate. Human pluripotent stem cells (hPSCs) provide a valuable source for deriving human hair cells to study their development and design therapies to treat and/or prevent their degeneration. In this study we used a dynamic 3D Rotary Cell Culture System (RCCS) for deriving inner ear organoids from hPSCs. We show RCCS-derived organoids recapitulate stages of inner ear development and give rise to an enriched population of hair cells displaying vestibular-like morphological and physiological phenotypes, which resemble developing human fetal inner ear hair cells as well as the presence of accessory otoconia-like structures. These results show that hPSC-derived organoids can generate complex inner ear structural features and be a resource to study inner ear development.
History
Publication title
Frontiers in Cell and Developmental BiologyVolume
7Article number
25Number
25ISSN
2296-634XDepartment/School
Menzies Institute for Medical ResearchPublisher
Frontiers Research FoundationPlace of publication
SwitzerlandRights statement
Copyright 2019 Mattei, Lim, Drury, Nasr, Li, Tadro, D'Abaco, Stok, Nayagam and Dottori. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/Repository Status
- Open