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Direct electrospinning of 3D auricle-shaped scaffolds for tissue engineering applications

Citation

Walser, J and Stok, KS and Caversaccio, MD and Ferguson, SJ, Direct electrospinning of 3D auricle-shaped scaffolds for tissue engineering applications, Biofabrication, 8, (2) pp. 025007. ISSN 1758-5082 (2016) [Refereed Article]

Copyright Statement

2016 IOP Publishing Ltd

DOI: doi:10.1088/1758-5090/8/2/025007

Abstract

Thirty-two poly(ε)caprolactone (PCL) scaffolds have been produced by electrospinning directly into an auricle-shaped mould and seeded with articular chondrocytes harvested from bovine ankle joints. After seeding, the auricle shaped constructs were cultured in vitro and analysed at days 1, 7, 14 and 21 for regional differences in total DNA, glycosaminoglycan (GAG) and collagen (COL) content as well as the expression of aggrecan (AGG), collagen type I and type II (COL1/2) and matrix metalloproteinase 3 and 13 (MMP3/13). Stress-relaxation indentation testing was performed to investigate regional mechanical properties of the electrospun constructs. Electrospinning into a conductive mould yielded stable 3D constructs both initially and for the whole in vitro culture period, with an equilibrium modulus in the MPa range. Rapid cell proliferation and COL accumulation was observed until week 3. Quantitative real time PCR analysis showed an initial increase in AGG, no change in COL2, a persistent increase in COL1, and only a slight decrease initially for MMP3. Electrospinning of fibrous scaffolds directly into an auricle-shape represents a promising option for auricular tissue engineering, as it can reduce the steps needed to achieve an implantable structure.

Item Details

Item Type:Refereed Article
Keywords:caprolactone, cartilage, chondrocytes, ear, electrospinning, tissue engineering
Research Division:Engineering
Research Group:Biomedical engineering
Research Field:Biomechanical engineering
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in engineering
UTAS Author:Stok, KS (Dr Kathryn Stok)
ID Code:133631
Year Published:2016
Web of Science® Times Cited:17
Deposited By:Menzies Institute for Medical Research
Deposited On:2019-07-04
Last Modified:2019-08-30
Downloads:0

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