eCite Digital Repository
Haem at the interface between pathogenic and commensal bacterial species in the human respiratory tract
Citation
Latham, RD and Del Rey, MT and Walshe, J and Atto, B and Guss, JM and Mackay, JP and Tristram, SG and Gell, DA, Haem at the interface between pathogenic and commensal bacterial species in the human respiratory tract, 2018 ComBio, 23-26 September, Sydney, NSW, pp. 37. (2018) [Conference Extract]
![]() | PDF Pending copyright assessment - Request a copy 2Mb |
Official URL: http://combio.org.au/combio2018
Abstract
Non-typeable Haemophilus influenzae (NTHi) is an important opportunistic pathogen of the human respiratory tract that has proved recalcitrant to vaccine development and shows increasing prevalence of antibiotic resistance, prompting us to search for alternative anti-microbial strategies. Haemophilus haemolyticus is closely related to NTHi, and also colonises the respiratory tract, but is recognised as a non-pathogenic commensal species. We identified two H. haemolyticusisolates secreting a 27-kDa protein that inhibited the growth of NTHi in vitro, but did not inhibit a range of other respiratory flora that were tested, suggesting a level of species-specificity against NTHi. A gene knockout established that the gene product of interest was responsible for inhibitory activity. Spectroscopic and x-ray crystallographic analysis of the recombinant protein identified a haem binding site. The protein shares structural features with some non-haem iron scavenging proteins, but the haem-binding site is unique. Insights into the biological function, including NTHi inhibitory actions, of this protein, based on structure, biochemistry and bioactivity assays are presented. The work is ongoing in the context that strains of H. haemolyticus might be developed as respiratory probiotics to combat colonisation and infection with NTHi.
Item Details
Item Type: | Conference Extract |
---|---|
Keywords: | haem, haemophilus |
Research Division: | Biomedical and Clinical Sciences |
Research Group: | Medical microbiology |
Research Field: | Medical bacteriology |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Latham, RD (Mr Roger Latham) |
UTAS Author: | Atto, B (Miss Brianna Atto) |
UTAS Author: | Tristram, SG (Dr Stephen Tristram) |
UTAS Author: | Gell, DA (Dr David Gell) |
ID Code: | 133603 |
Year Published: | 2018 |
Deposited By: | Health Sciences |
Deposited On: | 2019-07-03 |
Last Modified: | 2019-07-03 |
Downloads: | 0 |
Repository Staff Only: item control page