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High fat diet inhibits dendritic cell and T cell response to allergens but does not impair inhalational respiratory tolerance


Pizzolla, A and Oh, DY and Luong, S and Prickett, SR and Henstridge, DC and Febbraio, MA and O'Hehir, RE and Rolland, JM and Hardy, CL, High fat diet inhibits dendritic cell and T cell response to allergens but does not impair inhalational respiratory tolerance, PLoS ONE, 11, (8) pp. e0160407. ISSN 1932-6203 (2016) [Refereed Article]


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Copyright 2016 Pizzolla et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0)

DOI: doi:10.1371/journal.pone.0160407


The incidence of obesity has risen to epidemic proportions in recent decades, most commonly attributed to an increasingly sedentary lifestyle, and a 'western' diet high in fat and low in fibre. Although non-allergic asthma is a well-established co-morbidity of obesity, the influence of obesity on allergic asthma is still under debate. Allergic asthma is thought to result from impaired tolerance to airborne antigens, so-called respiratory tolerance. We sought to investigate whether a diet high in fats affects the development of respiratory tolerance. Mice fed a high fat diet (HFD) for 8 weeks showed weight gain, metabolic disease, and alteration in gut microbiota, metabolites and glucose metabolism compared to age-matched mice fed normal chow diet (ND). Respiratory tolerance was induced by repeated intranasal (i.n.) administration of ovalbumin (OVA), prior to induction of allergic airway inflammation (AAI) by sensitization with OVA in alum i.p. and subsequent i.n. OVA challenge. Surprisingly, respiratory tolerance was induced equally well in HFD and ND mice, as evidenced by decreased lung eosinophilia and serum OVA-specific IgE production. However, in a pilot study, HFD mice showed a tendency for impaired activation of airway dendritic cells and regulatory T cells compared with ND mice after induction of respiratory tolerance. Moreover, the capacity of lymph node cells to produce IL-5 and IL-13 after AAI was drastically diminished in HFD mice compared to ND mice. These results indicate that HFD does not affect the inflammatory or B cell response to an allergen, but inhibits priming of Th2 cells and possibly dendritic cell and regulatory T cell activation.

Item Details

Item Type:Refereed Article
Keywords:allergen, aluminum potassium sulfate, immunoglobulin E, interleukin 13, interleukin 5, ovalbumin, allergen, aluminum potassium sulfate, aluminum sulfate, fat intake, immunoglobulin E, interleukin 13, interleukin 5, ovalbumin, animal cell
Research Division:Biological Sciences
Research Group:Biochemistry and cell biology
Research Field:Cell metabolism
Objective Division:Health
Objective Group:Clinical health
Objective Field:Clinical health not elsewhere classified
UTAS Author:Henstridge, DC (Dr Darren Henstridge)
ID Code:133361
Year Published:2016
Web of Science® Times Cited:16
Deposited By:Health Sciences
Deposited On:2019-06-24
Last Modified:2022-08-25
Downloads:17 View Download Statistics

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