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Novel bilayer bacterial nanocellulose scaffold supports neocartilage formation in vitro and in vivo

Citation

Martinez Avila, H and Feldmann, E-M and Pleumeekers, MM and Nimeskern, L and Kuo, W and de Jong, WC and Schwarz, S and Muller, R and Hendriks, J and Rotter, N and van Osch, GJVM and Stok, KS and Gatenholm, P, Novel bilayer bacterial nanocellulose scaffold supports neocartilage formation in vitro and in vivo, Biomaterials, 44 pp. 122-133. ISSN 0142-9612 (2015) [Refereed Article]

Copyright Statement

2014 Elsevier Ltd. All rights reserved.

DOI: doi:10.1016/j.biomaterials.2014.12.025

Abstract

Tissue engineering provides a promising alternative therapy to the complex surgical reconstruction of auricular cartilage by using ear-shaped autologous costal cartilage. Bacterial nanocellulose (BNC) is proposed as a promising scaffold material for auricular cartilage reconstruction, as it exhibits excellent biocompatibility and secures tissue integration. Thus, this study evaluates a novel bilayer BNC scaffold for auricular cartilage tissue engineering. Bilayer BNC scaffolds, composed of a dense nanocellulose layer joined with a macroporous composite layer of nanocellulose and alginate, were seeded with human nasoseptal chondrocytes (NC) and cultured in vitro for up to 6 weeks. To scale up for clinical translation, bilayer BNC scaffolds were seeded with a low number of freshly isolated (uncultured) human NCs combined with freshly isolated human mononuclear cells (MNC) from bone marrow in alginate and subcutaneously implanted in nude mice for 8 weeks. 3D morphometric analysis showed that bilayer BNC scaffolds have a porosity of 75% and mean pore size of 50 25 μm. Furthermore, endotoxin analysis and in vitro cytotoxicity testing revealed that the produced bilayer BNC scaffolds were non-pyrogenic (0.15 0.09 EU/ml) and non-cytotoxic (cell viability: 97.8 4.7%). This study demonstrates that bilayer BNC scaffolds offer a good mechanical stability and maintain a structural integrity while providing a porous architecture that supports cell ingrowth. Moreover, bilayer BNC scaffolds provide a suitable environment for culture-expanded NCs as well as a combination of freshly isolated NCs and MNCs to form cartilage in vitro and in vivo as demonstrated by immunohistochemistry, biochemical and biomechanical analyses.

Item Details

Item Type:Refereed Article
Keywords:tissue engineering, ear cartilage, neo-cartilage, bacterial cellulose, nasoseptal chondrocytes, mononuclear cells
Research Division:Engineering
Research Group:Biomedical engineering
Research Field:Biomechanical engineering
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in engineering
UTAS Author:Stok, KS (Dr Kathryn Stok)
ID Code:133201
Year Published:2015
Web of Science® Times Cited:56
Deposited By:Menzies Institute for Medical Research
Deposited On:2019-06-18
Last Modified:2019-08-08
Downloads:0

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