eCite Digital Repository

Mechano-functional assessment of human mesenchymal stem cells grown in three-dimensional hyaluronan-based scaffolds for cartilage tissue engineering

Citation

Stok, KS and Lisignoli, G and Cristino, S and Facchini, A and Muller, R, Mechano-functional assessment of human mesenchymal stem cells grown in three-dimensional hyaluronan-based scaffolds for cartilage tissue engineering, Journal of Biomedical Materials Research - Part A, 93, (1) pp. 37-45. ISSN 1549-3296 (2009) [Refereed Article]


Preview
PDF
Pending copyright assessment - Request a copy
292Kb
  

DOI: doi:10.1002/jbm.a.32503

Abstract

Human mesenchymal stem cells (hMSCs) are an alternative cell source in bioconstruct production for cartilage regeneration, and hyaluronic acid (HA) is a widely-used bioabsorbable scaffold material used for cartilage regeneration. In this work, the aims were to evaluate the mechanical competency of hMSC-seeded HA scaffolds compared with native intact human articular cartilage, and in relation to its cellular properties. Human MSCs were grown under static conditions in HA scaffolds and then tested, in stepwise, stress-relaxation indentation, 7, 14, and 21 days later. Scaffolds at days 14 and 21 showed a significant increase in mechanical measures when compared with day 7 and unseeded scaffold material, but did not achieve the same levels as human cartilage. There was consistent stiffness within the scaffold, with a decreased stiffness around the edge. In vitro culture of hMSC-seeded HA scaffolds over 3 weeks produces a white, solid tissue compared with unseeded constructs. Increased cell proliferation and collagen type II expression were also seen over this period of time. These results demonstrate the competency of the neo-formed cartilagelike tissue in relation to its mechanical and cellular properties, and further, the importance, for future clinical use, of implanting this construct after 14 days of culture.

Item Details

Item Type:Refereed Article
Keywords:cartilage tissue engineering, mechanical properties, indentation testing, stress-relaxation, Hyaff-11, mesenchymal stem cells
Research Division:Engineering
Research Group:Biomedical Engineering
Research Field:Biomechanical Engineering
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in Engineering
UTAS Author:Stok, KS (Dr Kathryn Stok)
ID Code:133109
Year Published:2009
Web of Science® Times Cited:20
Deposited By:Menzies Institute for Medical Research
Deposited On:2019-06-12
Last Modified:2019-06-12
Downloads:0

Repository Staff Only: item control page