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MicroRNA-31 negatively regulates Interleukin-34 expression in vitro
journal contribution
posted on 2023-05-20, 04:09 authored by Li, M, Dong, Y, Chen, Z, Meng, L, Liu, X, Zhang, X, Wang, H, Mao, W, Zhang, J, Jiang, Z, Huang, T, Hu, J, Luo, P, Heinrich KornerHeinrich Korner, Ying, S, Li, JInterleukin-34 (IL-34) is a recently discovered cytokine that promotes tissue macrophage maturation and differentiation. We previously found that 1α,25-Dihydroxyvitamin D3 up-regulated IL-34 expression in SH-SY5Y neural cells. However, whether microRNA regulates IL-34 expression is not completely clear. By using on-line TargetScan and MiRanda software, we found that there was only one conserved microRNA-31 (miR-31) binding site in the 3' untranslated region (3'UTR) of IL-34 mRNA. Intriguingly, using qPCR we demonstrated that miR-31 levels were negatively correlated to IL-34 mRNA levels in different cell lines. By examining the effect of miR-31 on IL-34 3' UTR reporter luciferase activity and on IL-34 mRNA and argonaute RISC catalytic component 2 (AGO2) binding, it was found that miR-31 bound directly to IL-34 3'UTR and regulated the post-transcriptional expression of IL-34 in MGC-803 cells. Moreover, a miR-31 mimic significantly reduced IL-34 expression levels while a miR-31 inhibitor up-regulated IL-34 expression in KYSE-45 and HT-29 cells. Taken together, these results show that miR-31 negatively regulates IL-34 expression by directly binding to the IL-34 3' UTR in vitro.
History
Publication title
Immunological InvestigationsVolume
48Issue
6Pagination
597-607ISSN
0882-0139Department/School
Menzies Institute for Medical ResearchPublisher
Marcel Dekker IncPlace of publication
270 Madison Ave, New York, USA, Ny, 10016Rights statement
Copyright 2019 Taylor & Francis Group, LLCRepository Status
- Restricted