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MicroRNA-31 negatively regulates Interleukin-34 expression in vitro

Citation

Li, M and Dong, Y and Chen, Z and Meng, L and Liu, X and Zhang, X and Wang, H and Mao, W and Zhang, J and Jiang, Z and Huang, T and Hu, J and Luo, P and Korner, H and Ying, S and Li, J, MicroRNA-31 negatively regulates Interleukin-34 expression in vitro, Immunological Investigations pp. 1-11. ISSN 0882-0139 (2019) [Refereed Article]

Copyright Statement

Copyright 2019 Taylor & Francis Group, LLC

DOI: doi:10.1080/08820139.2019.1578230

Abstract

Interleukin-34 (IL-34) is a recently discovered cytokine that promotes tissue macrophage maturation and differentiation. We previously found that 1α,25-Dihydroxyvitamin D3 up-regulated IL-34 expression in SH-SY5Y neural cells. However, whether microRNA regulates IL-34 expression is not completely clear. By using on-line TargetScan and MiRanda software, we found that there was only one conserved microRNA-31 (miR-31) binding site in the 3' untranslated region (3'UTR) of IL-34 mRNA. Intriguingly, using qPCR we demonstrated that miR-31 levels were negatively correlated to IL-34 mRNA levels in different cell lines. By examining the effect of miR-31 on IL-34 3' UTR reporter luciferase activity and on IL-34 mRNA and argonaute RISC catalytic component 2 (AGO2) binding, it was found that miR-31 bound directly to IL-34 3'UTR and regulated the post-transcriptional expression of IL-34 in MGC-803 cells. Moreover, a miR-31 mimic significantly reduced IL-34 expression levels while a miR-31 inhibitor up-regulated IL-34 expression in KYSE-45 and HT-29 cells. Taken together, these results show that miR-31 negatively regulates IL-34 expression by directly binding to the IL-34 3' UTR in vitro.

Item Details

Item Type:Refereed Article
Keywords:IL-34, MiR-31, MicroRNA, regulation
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Cellular Immunology
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Infectious Diseases
UTAS Author:Korner, H (Professor Heinrich Korner)
ID Code:133074
Year Published:2019
Web of Science® Times Cited:3
Deposited By:Menzies Institute for Medical Research
Deposited On:2019-06-06
Last Modified:2019-08-20
Downloads:0

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