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Flavobacterium hibernum sp. nov., a lactose-utilizing bacterium from a freshwater Antarctic lake

Citation

McCammon, SA and Innes, BH and Bowman, JP and Franzmann, PD and Dobson, SJ and Holloway, PE and Skerratt, J and Nichols, PD and Rankin, LM, Flavobacterium hibernum sp. nov., a lactose-utilizing bacterium from a freshwater Antarctic lake, International Journal of Systematic Bacteriology, 48, (4) pp. 1405-1412. ISSN 0020-7713 (1998) [Refereed Article]

DOI: doi:10.1099/00207713-48-4-1405

Abstract

Four freshwater Antarctic lakes were examined for the presence of β- galactosidase-producing bacteria using mineral medium enrichments and lactose. Enrichments from only one of the lakes produced growth and two strains were isolated that were very similar in phenotype and fatty acid profile, and shared considerable homology in their DNA (DNA-DNA hybridization = 93±7%). The strains were psychrotrophic with theoretical T(max), T(min), and T(opt) of 30-31, -7 °and 26 °C, respectively. The β-galactosidase in cell extracts had an optimal activity at 39 °C. The strains were Gram- negative rods, showed gliding motility, contained branched and hydroxy fatty acids, and menaquinone 6 as the major respiratory quinone. The strains did not form microcysts and utilized lactose while using ammonium ions as a source of nitrogen, and a range of other sugars. The G+C content of the DNA was 34 mol%. Phylogenetic analysis of one of the strains, by comparison of 16S rDNA sequences, showed that it was most similar, but not identical to, Flavobacterium columnare and '[Sporocytophaga] cauliformis'. Both species could be differentiated phenotypically from the Antarctic isolates. DNA-DNA hybridization of the Antarctic isolate with six different members of the Flavobacterium 16S rDNA cluster showed no strain with greater than 18% relatedness. The nearest type species to the Antarctic isolate in the phylogenetic analysis was Flavobacterium aquatile. The name Flavobacterium hibernum is proposed for the Antarctic strains, and the type strain is ATCC 51468(T) (= ACAM 376(T)).

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Bacteriology
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:McCammon, SA (Ms Sharee McCammon)
Author:Innes, BH (Ms Bronwyn Innes)
Author:Bowman, JP (Associate Professor John Bowman)
Author:Dobson, SJ (Dr Susan Dobson)
Author:Holloway, PE (Dr Paul Holloway)
Author:Skerratt, J (Dr Jenny Skerratt)
Author:Nichols, PD (Dr Peter Nichols)
Author:Rankin, LM (Ms Lynne Maree Rankin)
ID Code:13301
Year Published:1998
Web of Science® Times Cited:61
Deposited By:Agricultural Science
Deposited On:1998-08-01
Last Modified:2011-08-08
Downloads:0

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