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Determination of glyoxal and methylglyoxal in serum by UHPLC coupled with fluorescence detection
Citation
Dhananjayan, K and Irrgang, F and Raju, R and Harman, DG and Moran, C and Srikanth, V and Munch, G, Determination of glyoxal and methylglyoxal in serum by UHPLC coupled with fluorescence detection, Analytical Biochemistry, 573 pp. 51-66. ISSN 0003-2697 (2019) [Refereed Article]
Copyright Statement
Copyright 2019 Published by Elsevier Inc.
DOI: doi:10.1016/j.ab.2019.02.014
Abstract
Glyoxal (GO) and methylglyoxal (MGO) are two important biomarkers in diabetes. Analytical methods for determination of GO and MGO in serum samples are either HPLC with UV-Vis (low sensitivity) or MS/MS (expensive) detection. These disadvantages have hampered the introduction of these biomarkers as a routine analyte for diabetes diagnostics into the clinical laboratory. In this study, we introduce a UHPLC method with fluorescence detection for the measurement of GO and MGO in serum samples by pre-column derivatization at neutral pH with 5, 6-diamino-2,4-dihydroxypyrimidine sulfate (DDP) to form lumazines. The method was validated as per FDA guidelines. Using this method, we have determined GO and MGO in a variety of animal serum samples, and for example, determined the GO and MGO concentration in adult bovine serum to be 852 ± 27 and 192 ± 10 nmol/L, respectively. In human serum, GO and MGO levels in non-diabetic subjects (n = 14) were determined to be 154 ± 88 and 98 ± 27 nmol/L, and in serum samples from subjects with diabetes (n = 14) 244 ± 137 and 190 ± 68 nmol/L, respectively. In addition, interference studies showed that physiological serum components did not lead to an artificial increase in the levels of GO and MGO.
Item Details
Item Type: | Refereed Article |
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Keywords: | blood samples, fluorescence, glyoxal, methylglyoxal, UHPLC, validation |
Research Division: | Biological Sciences |
Research Group: | Biochemistry and cell biology |
Research Field: | Analytical biochemistry |
Objective Division: | Health |
Objective Group: | Clinical health |
Objective Field: | Clinical health not elsewhere classified |
UTAS Author: | Srikanth, V (Dr Velandai Srikanth) |
ID Code: | 132981 |
Year Published: | 2019 |
Web of Science® Times Cited: | 12 |
Deposited By: | Menzies Institute for Medical Research |
Deposited On: | 2019-05-29 |
Last Modified: | 2020-08-14 |
Downloads: | 0 |
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