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Effect of protein synthesis inhibitor cycloheximide on starvation, fasting and feeding oxygen consumption in juvenile spiny lobster Sagmariasus verreauxi

Citation

Wang, S and Fitzgibbon, QP and Carter, CG and Smith, GG, Effect of protein synthesis inhibitor cycloheximide on starvation, fasting and feeding oxygen consumption in juvenile spiny lobster Sagmariasus verreauxi, Journal of Comparative Physiology B, 189, (3-4) pp. 351-365. ISSN 0174-1578 (2019) [Refereed Article]

Copyright Statement

Copyright 2019 Springer-Verlag GmbH Germany, part of Springer Nature

DOI: doi:10.1007/s00360-019-01221-z

Abstract

Metabolism in aquatic ectotherms evaluated by oxygen consumption rates reflects energetic costs including those associated with protein synthesis. Metabolism is influenced by nutritional status governed by feeding, nutrient intake and quality, and time without food. However, little is understood about contribution of protein synthesis to crustacean energy metabolism. This study is the first using a protein synthesis inhibitor cycloheximide to research contribution of cycloheximide-sensitive protein synthesis to decapod crustacean metabolism. Juvenile Sagmariasus verreauxi were subject to five treatments: 2-day fasted lobsters sham injected with saline; 2-day fasted lobsters injected with cycloheximide; 10-day starved lobsters injected with cycloheximide; post-prandial lobsters fed with squid Nototodarus sloanii with no further treatment; and post-prandial lobsters injected with cycloheximide. Standard and routine metabolic rates in starved lobsters were reduced by 32% and 41%, respectively, compared to fasted lobsters, demonstrating metabolic downregulation with starvation. Oxygen consumption rates of fasted and starved lobsters following cycloheximide injection were reduced by 29% and 13%, respectively, demonstrating protein synthesis represents only a minor component of energy metabolism in unfed lobsters. Oxygen consumption rate of fed lobsters was reduced by 96% following cycloheximide injection, demonstrating protein synthesis in decapods contributes a major proportion of specific dynamic action (SDA). SDA in decapods is predominantly a post-absorptive process likely related to somatic growth. This work extends previously limited knowledge on contribution of protein synthesis to crustacean metabolism, which is crucial to explore the relationship between nutritional status and diet quality and how this will affect growth potential in aquaculture species.

Item Details

Item Type:Refereed Article
Keywords:Sagmariasus verreauxi, oxygen consumption, energetic costs, protein synthesis, cycloheximide
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Aquaculture rock lobster
UTAS Author:Wang, S (Mr Shuangyao Wang)
UTAS Author:Fitzgibbon, QP (Associate Professor Quinn Fitzgibbon)
UTAS Author:Carter, CG (Professor Chris Carter)
UTAS Author:Smith, GG (Professor Gregory Smith)
ID Code:132746
Year Published:2019
Funding Support:Australian Research Council (IH120100032)
Web of Science® Times Cited:5
Deposited By:Fisheries and Aquaculture
Deposited On:2019-05-20
Last Modified:2020-01-14
Downloads:0

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