Thaver, S and Bennett, EJ and Foa, L and Richards, SM and Lyons, AB and Zosky, GR, Pregnancy protects against the pro-inflammatory respiratory responses induced by particulate matter exposure, Chemosphere, 225 pp. 796-802. ISSN 0045-6535 (2019) [Refereed Article]
© 2019 Elsevier Ltd
Methods: Pregnant, eight-week-old C57BL/6J mice were exposed intranasally to 50 μg of diesel exhaust particles (DEP), iron oxide (Fe2O3) or silica (SiO2) in 50 μL of saline, or saline alone, on gestational day (E)7.5, E12.5 and E17.5. Groups of non-pregnant mice were exposed on day (D)0, D5 and D10. Biological samples were collected 24 h after the last exposure. Serum IL-4 and IL-6 levels were quantified by ELISA. Bronchoalveolar lavage (BAL) fluid was collected for inflammatory cells counts and assessment of IFN-ɣ, IL-4, IL-5, IL-6, IL-8 and IL-10 levels by ELISA. The spleen and thymus were also collected and the percentage of B cells and CD4+, CD8+ and CD4+CD25 + T cells were determined by flow cytometry.
Results: Exposure to silica caused an influx of lymphocytes, eosinophils and neutrophils into the lung. The magnitude of this response was suppressed by pregnancy. Pregnancy also enhanced the production of CD4+CD25 + T cells in response to DEP and silica exposure.
Conclusions: Collectively, our data suggest that pregnancy reduces the inflammatory response to silica and alters the immune response to DEP. These responses were accompanied by pregnancy related changes including increased IL-4 production, reduced IL-8 production and an increase in the proportion of CD4+CD25 + T cells in response to PM exposure.
|Item Type:||Refereed Article|
|Keywords:||pregnancy, particulate matter, inflammation, immune cells|
|Research Division:||Biological Sciences|
|Research Field:||Animal Immunology|
|Objective Group:||Clinical Health (Organs, Diseases and Abnormal Conditions)|
|Objective Field:||Immune System and Allergy|
|UTAS Author:||Thaver, S (Mr Santon Thaver)|
|UTAS Author:||Bennett, EJ (Ms Ellen Bennett)|
|UTAS Author:||Foa, L (Professor Lisa Foa)|
|UTAS Author:||Richards, SM (Dr Stephen Richards)|
|UTAS Author:||Lyons, AB (Associate Professor Bruce Lyons)|
|UTAS Author:||Zosky, GR (Professor Graeme Zosky)|
|Deposited By:||Menzies Institute for Medical Research|
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