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Functional analysis of a Drosophila melanogaster olfactory receptor expressed in Sf9 cells

Citation

Kiely, A and Authier, A and Kralicek, AV and Warr, CG and Newcombe, RD, Functional analysis of a Drosophila melanogaster olfactory receptor expressed in Sf9 cells, Journal of Neuroscience Methods, 159, (2) pp. 189-194. ISSN 0165-0270 (2007) [Refereed Article]

DOI: doi:10.1016/j.jneumeth.2006.07.005

Abstract

Olfactory receptors (ORs) are seven transmembrane proteins that are responsible for the transduction of volatiles into neuronal signals. Their low sequence homology means that the prediction of ligands for ORs based on extrapolation from empirical data of other ORs is difficult, so an experimental approach must be used. Here, we report a functional assay for insect ORs using calcium-imaging in Sf9 cells. We find that the interaction of the odorant, ethyl butyrate, with the Drosophila melanogaster olfactory receptor Or22a is both dose-dependent and highly sensitive, with Or22a responding to ethyl butyrate with an EC(50) of (1.58+/-0.82)x10(-11)M. This degree of sensitivity does not require the addition of odorant binding proteins or downstream signal transduction elements. Furthermore, we demonstrate that Or22a expressed in Sf9 cells has a similar response profile to a range of odorants previously tested in vivo. This functional assay system will provide a useful tool for the de-orphaning of ORs from a wide range of insect species that are yet to have ligands assigned, and will help provide insight into OR specificity and mechanism of activation.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Neurogenetics
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
UTAS Author:Warr, CG (Professor Coral Warr)
ID Code:131978
Year Published:2007
Web of Science® Times Cited:48
Deposited By:Office of the School of Medicine
Deposited On:2019-04-16
Last Modified:2019-04-16
Downloads:0

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