Bermudez, R and Losada, AP and de Azevedo, AM and Guerra-Varela, J and Perez-Fernandez, D and Sanchez, L and Padros, F and Nowak, B and Quiroga, MI, First description of a natural infection with spleen and kidney necrosis virus in zebrafish, Journal of Fish Diseases, 41, (8) pp. 1283-1294. ISSN 0140-7775 (2018) [Refereed Article]
Copyright 2018 John Wiley & Sons Ltd.
Zebrafish has become a popular research model in the last years, and several diseases affecting zebrafish research facilities have been reported. However, only one case of naturally occurring viral infections was described for this species. In 2015, infectious spleen and kidney necrosis virus (ISKNV) was detected in zebrafish from a research facility in Spain. Affected fish showed lethargy, loss of appetite, abnormal swimming, distention of the coelomic cavity and, in the most severe cases, respiratory distress, pale gills and petechial haemorrhages at the base of fins. Cytomegaly was the most relevant histopathological finding in organs and tissues, sometimes associated to degenerative and necrotic changes. ISKNV belongs to the relatively newly defined genus Megalocytivirus, family Iridoviridae, comprising large, icosahedral cytoplasmic DNA viruses. This is the first case of naturally occurring Megalocytivirus infection in zebrafish research facilities, associated with morbidity. The virus has been identified based on both pathologic and genetic evidence, to better understand the pathogenesis of the infection in zebrafish and the phylogenetic relationship with other iridoviruses. Given the ability of megalocytiviruses to cross‐species boundaries, it seems necessary to implement stringent biosecurity practices as these infections may invalidate experimental data and have major impact on laboratory and cultured fish.
|Item Type:||Refereed Article|
|Keywords:||megalocytivirus, natural infection, pathology, zebrafish|
|Research Division:||Biological Sciences|
|Research Field:||Genetic Immunology|
|Objective Division:||Expanding Knowledge|
|Objective Group:||Expanding Knowledge|
|Objective Field:||Expanding Knowledge in the Biological Sciences|
|UTAS Author:||Nowak, B (Professor Barbara Nowak)|
|Web of Science® Times Cited:||6|
|Deposited By:||Fisheries and Aquaculture|
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