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Trunk cleavage is essential for Drosophila terminal patterning and can occur independently of Torso-like

Citation

Henstridge, MA and Johnson, TK and Warr, CG and Whisstock, JC, Trunk cleavage is essential for Drosophila terminal patterning and can occur independently of Torso-like, Nature Communications, 5 pp. 1-7. ISSN 2041-1723 (2014) [Refereed Article]

Copyright Statement

Copyright 2014 Macmillan Publishers Limited

DOI: doi:10.1038/ncomms4419

Abstract

Terminal patterning in Drosophila is governed by a localized interaction between the Torso kinase (Tor) and its ligand Trunk (Trk). Currently, it is proposed that Trk must be cleaved in order to bind Tor, and that these proteolytic events are controlled by secretion of Torso-like (Tsl) only at the embryo poles. However, controversy surrounds these ideas since neither cleaved Trk nor a protease that functions in terminal patterning have been identified. Here we show that Trk is cleaved multiple times in vivo and that these proteolytic events are essential for its function. Unexpectedly, however, the Trk cleavage patterns we observe are unaltered in tsl-null mutants. One explanation for these data is that the influence of Tsl on localized Trk cleavage at the embryo poles is subtle and cannot be readily detected. Alternatively, we favour a scenario where Tsl functions post proteolytic processing of Trk to control localized terminal patterning.

Item Details

Item Type:Refereed Article
Keywords:noggin; protein tyrosine kinase; torso kinase; unclassified drug; Drosophila protein; protein tyrosine kinase; torso protein, Drosophila; Torso-like protein, Drosophila; trunk protein, Drosophila; anatomy; embryo; fly; proteomics; secretion; adult
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Developmental Genetics (incl. Sex Determination)
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Biological Sciences
UTAS Author:Warr, CG (Professor Coral Warr)
ID Code:131823
Year Published:2014
Web of Science® Times Cited:17
Deposited By:Office of the School of Medicine
Deposited On:2019-04-08
Last Modified:2019-05-31
Downloads:0

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