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The Drosophila melanogaster Phospholipid Flippase dATP8B Is Required for Odorant Receptor Function

Citation

Liu, Y-C and Pearce, MW and Honda, T and Johnson, TK and Charlu, S and Sharma, KR and Imad, M and Burke, RE and Zinsmaier, KE and Ray, A and Dahanukar, A and de Bruyne, M and Warr, CG, The Drosophila melanogaster Phospholipid Flippase dATP8B Is Required for Odorant Receptor Function, PLoS Genetics, 10, (3) pp. 1-9. ISSN 1553-7390 (2014) [Refereed Article]


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Copyright 2014 Liu et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1371/journal.pgen.1004209

Abstract

The olfactory systems of insects are fundamental to all aspects of their behaviour, and insect olfactory receptor neurons (ORNs) exhibit exquisite specificity and sensitivity to a wide range of environmental cues. In Drosophila melanogaster, ORN responses are determined by three different receptor families, the odorant (Or), ionotropic-like (IR) and gustatory (Gr) receptors. However, the precise mechanisms of signalling by these different receptor families are not fully understood. Here we report the unexpected finding that the type 4 P-type ATPase phospholipid transporter dATP8B, the homologue of a protein associated with intrahepatic cholestasis and hearing loss in humans, is crucial for Drosophila olfactory responses. Mutations in dATP8B severely attenuate sensitivity of odorant detection specifically in Or-expressing ORNs, but do not affect responses mediated by IR or Gr receptors. Accordingly, we find dATP8B to be expressed in ORNs and localised to the dendritic membrane of the olfactory neurons where signal transduction occurs. Localisation of Or proteins to the dendrites is unaffected in dATP8B mutants, as is dendrite morphology, suggesting instead that dATP8B is critical for Or signalling. As dATP8B is a member of the phospholipid flippase family of ATPases, which function to determine asymmetry in phospholipid composition between the outer and inner leaflets of plasma membranes, our findings suggest a requirement for phospholipid asymmetry in the signalling of a specific family of chemoreceptor proteins.

Item Details

Item Type:Refereed Article
Keywords:olfactory marker protein; ATP8B protein, Drosophila; Drosophila protein; olfactory receptor; phospholipid transfer protein; action potential; adult; animal experiment; animal tissue; article; cell structure; controlled study; dATP8B gene; dendrite
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Neurogenetics
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Warr, CG (Professor Coral Warr)
ID Code:131821
Year Published:2014
Web of Science® Times Cited:13
Deposited By:Medicine
Deposited On:2019-04-08
Last Modified:2019-05-01
Downloads:29 View Download Statistics

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