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Development of hematological respiratory variables in late chicken embryos: The relative importance of incubation time and embryo mass

Citation

Tazawa, H and Andrewartha, SJ and Burggren, WW, Development of hematological respiratory variables in late chicken embryos: The relative importance of incubation time and embryo mass, Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, 159, (3) pp. 225-233. ISSN 1095-6433 (2011) [Refereed Article]

Copyright Statement

2011 Published by Elsevier Inc.

DOI: doi:10.1016/j.cbpa.2011.02.024

Abstract

Oxygen demand increases during embryonic development, requiring an increase in red blood cells (RBCs) containing hemoglobin (Hb) to transport O2 between the respiratory organ and systemic tissues. A thorough ontogenetic understanding of the onset and maturation of the complex regulatory processes for RBC concentration ([RBC]), Hb concentration ([Hb]), hematocrit (Hct), mean corpuscular indices (mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration ([MCHb])) is currently lacking. We hypothesize that during the last half of incubation when the respiratory organ (the chorioallantoic membrane) envelops most of the egg contents, mean corpuscular indices will stabilize. Accordingly, Hct, [RBC] and [Hb] must also all change proportionally across development. Further, we hypothesize that the hematological respiratory variables develop and mature as a function of incubation duration, independently of embryonic growth. As predicted, a similar increase in Hct (from 18.70.6% on day 10 (d10) to 34.10.5% on d19 of incubation), [RBC] (1.130.03נ106/μL to 2.500.03נ106/μL) and [Hb] (6.10.2g% to 11.20.1g%) occurred during d1019. Both [RBC] and [Hb] demonstrated high linear correlation with Hct, resulting in constant [MCHb] (~33g% from d10 to d19). The decrease in MCV (from ~165μ3 on d10 to ~140μ3 on d13) and MCH (~55pg to ~ 45pg) during d1013, may be attributed to a changeover from larger primary to smaller secondary and adult-type erythrocytes with MCV and MCH remaining constant (~140μ3 and ~45pg respectively) for the rest of the incubation period (d1319). Hematological respiratory values on a given incubation day were identical between embryos of different masses using either natural mass variation or experimental growth acceleration, indicating that the hematological variables develop as a function of incubation time, irrespective of embryo growth.

Item Details

Item Type:Refereed Article
Keywords:body mass, developmental patterns, growth acceleration Hct, [RBC] and [Hb], mean corpuscular indices (MCV, MCH, [MCHb]), natural mass variation
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Animal developmental and reproductive biology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Andrewartha, SJ (Dr Sarah Andrewartha)
ID Code:131602
Year Published:2011
Web of Science® Times Cited:18
Deposited By:Agriculture and Food Systems
Deposited On:2019-03-26
Last Modified:2019-04-26
Downloads:0

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